College of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China.
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China.
Genes (Basel). 2023 Jan 4;14(1):138. doi: 10.3390/genes14010138.
Catalase (CAT) is an important antioxidant enzyme in plants that plays a key role in plant growth and stress responses. CAT is usually encoded by a small gene family that has been cloned and functionally studied in some species, such as , wheat and cucumber, but its specific roles in rice are not clear at present. In this study, we identified three CAT family genes (, and ) in the rice genome and performed a systematic bioinformatics analysis. RT-PCR analysis revealed that was primarily expressed in vegetative tissues such as roots, stems and leaves. Since showed the highest expression level among the three genes, we then focused on its related functions. OsCAT3 prokaryotic expression protein has an obvious ability to remove HO. The plant was short and had a low survival rate, the leaves were small with brown lesions, and the activities of the CAT, POD and SOD enzymes were significantly reduced. A microarray analysis showed that differentially expressed genes were primarily enriched in toxin metabolism and photosynthesis. This study laid a foundation for further understanding the function of the rice gene.
过氧化氢酶 (CAT) 是植物中一种重要的抗氧化酶，在植物生长和应激反应中发挥关键作用。CAT 通常由一个小的基因家族编码，该基因家族已在一些物种中被克隆并进行了功能研究，如 、小麦和黄瓜，但目前其在水稻中的具体作用尚不清楚。在这项研究中，我们在水稻基因组中鉴定了三个 CAT 家族基因（、和），并进行了系统的生物信息学分析。RT-PCR 分析表明，主要在根、茎和叶等营养组织中表达。由于在三个 基因中表达水平最高，我们随后专注于其相关功能。OsCAT3 原核表达蛋白具有明显清除 HO 的能力。植物矮小，存活率低，叶片小且有棕色斑点，CAT、POD 和 SOD 酶的活性显著降低。微阵列分析表明，差异表达基因主要富集在毒素代谢和光合作用中。这项研究为进一步了解水稻 基因的功能奠定了基础。
