Ye Lei, Ji Xuefei, Song Zijian, Guan Liao, Zhao Liang, Wang Wenwen, Du Weidong
Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei 230022, China.
Department of Orthopaedics, Xuzhou Municipal First People's Hospital, Daxue Road 269, Xuzhou 221116, China.
Diagnostics (Basel). 2023 Jan 4;13(2):187. doi: 10.3390/diagnostics13020187.
Post-neurosurgical bacterial meningitis (PNBM) is one of the severe complications in patients receiving neurosurgical procedures. Recent studies have found microbe-related glycans play important roles in adhesion, invasion, and toxicity toward innate immunological reactions. In this study, we aimed to investigate the glycomic profile and its potential diagnostic efficacy in post-neurosurgical bacterial meningitis (PNBM) patients with hemorrhagic stroke. A total of 136 cerebrospinal fluid (CSF) samples were recruited and divided into a PNBM group and a non-PNBM group based on the clinical diagnostic criteria. A lectin biochip-based method was established for the detection of glycans in CSF. The clinicopathological data and biochemical parameters in CSF from all patients were analyzed. Two models for multivariate analysis investigating glycan changes in the CSF were conducted, aiming at determining the specific expression and diagnostic efficacy of lectin-probing glycans (LPGs) for PNBM. In univariate analysis, we found that 8 out of 11 LPGs were significantly correlated with PNBM. Model 1 multivariate analysis revealed that PNA (p = 0.034), Jacalin (p = 0.034) and LTL (p = 0.001) were differentially expressed in the CSF of PNBM patients compared with those of non-PNBM patients. Model 2 multivariate analysis further disclosed that LTL (p = 0.021) and CSF glucose (p < 0.001) had independent diagnostic efficacies in PNBM, with areas under the curve (AUC) of 0.703 and 0.922, respectively. In summary, this study provided a new insight into the subject of CSF glycomics concerning bacterial infection in patients with hemorrhagic stroke.
神经外科术后细菌性脑膜炎（PNBM）是接受神经外科手术患者的严重并发症之一。最近的研究发现，微生物相关聚糖在对先天免疫反应的粘附、侵袭和毒性方面发挥着重要作用。在本研究中，我们旨在调查出血性中风的神经外科术后细菌性脑膜炎（PNBM）患者的糖组学特征及其潜在诊断效能。共收集了136份脑脊液（CSF）样本，并根据临床诊断标准分为PNBM组和非PNBM组。建立了一种基于凝集素生物芯片的方法来检测脑脊液中的聚糖。分析了所有患者脑脊液中的临床病理数据和生化参数。进行了两个多变量分析模型，以研究脑脊液中聚糖的变化，旨在确定凝集素探测聚糖（LPGs）对PNBM的特异性表达和诊断效能。在单变量分析中，我们发现11种LPGs中有8种与PNBM显著相关。模型1多变量分析显示，与非PNBM患者相比，PNBM患者脑脊液中PNA（p = 0.034）、扁豆凝集素（Jacalin，p = 0.034）和麦芽凝集素（LTL，p = 0.001）差异表达。模型2多变量分析进一步表明，LTL（p = 0.021）和脑脊液葡萄糖（p < 0.001）在PNBM中具有独立的诊断效能，曲线下面积（AUC）分别为0.703和0.922。总之，本研究为出血性中风患者细菌性感染的脑脊液糖组学研究提供了新的见解。
