Zhao Liang, Li Pingping, Xu Ziao, Ji Xuefei, Guan Liao, Wang Xiaojian, Luo Jin, Cheng Hongwei, Ye Lei
Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Front Neurol. 2023 Jun 20;14:1166598. doi: 10.3389/fneur.2023.1166598. eCollection 2023.
Post-neurosurgical bacterial meningitis (PNBM) is a serious complication for patients who receive neurosurgical treatment, but the diagnosis is difficult given the complicated microenvironment orchestrated by sterile brain injury and pathogenic infection. In this study, we explored potential diagnostic biomarkers and immunological features using a proteomics platform.
A total of 31 patients with aneurysmal subarachnoid hemorrhage (aSAH) who received neurosurgical treatment were recruited for this study. Among them, 15 were diagnosed with PNBM. The remaining 16 patients were categorized into the non-PNBM group. Proteomics analysis of the cerebrospinal fluid (CSF) was conducted on the Olink platform, which contained 92 immunity-related molecules.
We found that the expressions of 27 CSF proteins were significantly different between the PNBM and non-PNBM groups. Of those 27 proteins, 15 proteins were upregulated and 12 were downregulated in the CSF of the PNBM group. The receiver operating characteristic curve analysis indicated that three proteins (pleiotrophin, CD27, and angiopoietin 1) had high diagnostic accuracy for PNBM. Furthermore, we also performed bioinformatics analysis to explore potential pathways and the subcellular localization of the proteins.
In summary, we found a cohort of immunity-related molecules that can serve as potential diagnostic biomarkers for PNBM in patients with aSAH. These molecules also provide an immunological profile of PNBM.
神经外科术后细菌性脑膜炎（PNBM）是接受神经外科治疗患者的一种严重并发症，但鉴于无菌性脑损伤和病原体感染所构成的复杂微环境，其诊断较为困难。在本研究中，我们使用蛋白质组学平台探索潜在的诊断生物标志物和免疫特征。
本研究招募了31例接受神经外科治疗的动脉瘤性蛛网膜下腔出血（aSAH）患者。其中，15例被诊断为PNBM。其余16例患者被归入非PNBM组。在包含92种免疫相关分子的Olink平台上对脑脊液（CSF）进行蛋白质组学分析。
我们发现PNBM组和非PNBM组之间有27种CSF蛋白的表达存在显著差异。在这27种蛋白中，PNBM组CSF中有15种蛋白上调，12种蛋白下调。受试者工作特征曲线分析表明，三种蛋白（多效生长因子、CD27和血管生成素1）对PNBM具有较高的诊断准确性。此外，我们还进行了生物信息学分析以探索潜在的信号通路和蛋白的亚细胞定位。
总之，我们发现了一组免疫相关分子，它们可作为aSAH患者PNBM的潜在诊断生物标志物。这些分子也提供了PNBM的免疫特征。
