Abraham E C, Abraham A, Kasten-Jolly J
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-3331.
Hemoglobin. 1987;11(4):329-39. doi: 10.3109/03630268709042852.
The minor components of Hb Bart's were separated by CM-cellulose chromatography, reverse-phase HPLC, and DEAE-cellulose chromatography. These were characterized by amino acid analysis, tryptic peptide analysis by HPLC, electrophoresis, and carbohydrate and phosphate analysis. Acetylated and glycated components of Hb Bart's were present in cord blood red cells. The ratio of gamma Ic/total gamma in Hb Bart's was nearly the same as that of Hb FIc/Hb F which may indicate that the gamma chain after its release from the ribosome is not a better acetylation substrate than Hb Fo. Glycation of the free gamma-chains seems to take place in vivo as readily as the major Hb components.
通过羧甲基纤维素色谱法、反相高效液相色谱法和二乙氨基乙基纤维素色谱法分离了血红蛋白巴氏的次要成分。通过氨基酸分析、高效液相色谱法的胰蛋白酶肽分析、电泳以及碳水化合物和磷酸盐分析对这些成分进行了表征。血红蛋白巴氏的乙酰化和糖化成分存在于脐血红细胞中。血红蛋白巴氏中γIc/总γ的比例与血红蛋白FIc/血红蛋白F的比例几乎相同，这可能表明γ链从核糖体释放后，其作为乙酰化底物并不比血红蛋白Fo更好。游离γ链的糖化在体内似乎与主要血红蛋白成分一样容易发生。
