Dhansu Pooja, Ram Bakshi, Singh Ashish Kumar, Tomar Sudhir Kumar, Karuppaiyan Ramaiyan, Kumar Ravinder, Chhabra Manohar Lal, Singh Ajay, Raja Arun Kumar, Kaushik Prashant, Pandey Shashi Kant
ICAR-Sugarcane Breeding Institute, Regional Center, Karnal 132001, Haryana, India.
ICAR-Sugarcane Breeding Institute, Coimbatore 641007, Tamil Nadu, India.
Foods. 2023 Jan 9;12(2):311. doi: 10.3390/foods12020311.
This investigation aimed to optimize the time, pH, pressure, and temperature of sugarcane juice pasteurization and to develop a "ready to serve" bottled sugarcane juice with a high preservation efficiency. Fresh sugarcane juice was extracted from sugarcane genotype Co 89003, and beverage samples were collected using three different treatments: sulphitation of juice with the addition of potassium metabisulphite (KMS-25, 50, 100, and 150 ppm), acidification of juice (addition of citric acid, to reduce the pH of the juice to 4.8, 4.5, and 4.25), and steam treatment of the canes (5 min, 10, and 15 min at 7 psi). In all treatments, the juice was pasteurized in glass bottles @ 65 °C for 25 min and stored at low temperature (5 °C) in pre-sterilized glass bottles. Juice properties such as the ˚Brix, total sugar, pH, and total phenolic content decreased with storage, whereas the microbial count, titrable acidity, and reducing sugar content significantly increased during storage. The addition of KMS, citric acid, and the steam treatment reduced the browning of juice and maintained the color of juice during storage, by inhibiting the polyphenol oxidase enzyme activity, from 0.571 unit/mL to 0.1 unit/mL. Among the selected treatments, sugarcane juice with KMS (100 and 150 ppm) and steam treatment of the canes for 5 and 10 min at 7 psi showed the minimum changes in physico-chemical properties, sensory qualities, and restricted microbial growth. Thesulphitation treatment with pasteurization proved best for increasing the shelf life of sugarcane juice upto 90 days with refrigeration. Similarly, the steam-subjected cane juice (10 and 15 min at 7 psi) could be effectively preserved for upto 30 days with refrigeration, without any preservative.
本研究旨在优化甘蔗汁巴氏杀菌的时间、pH值、压力和温度,并开发一种具有高保鲜效率的“即饮型”瓶装甘蔗汁。从甘蔗品种Co 89003中提取新鲜甘蔗汁,并采用三种不同处理方式收集饮料样品:添加焦亚硫酸钾(KMS - 25、50、100和150 ppm)对果汁进行亚硫酸化处理、对果汁进行酸化处理(添加柠檬酸,将果汁pH值降至4.8、4.5和4.25)以及对甘蔗进行蒸汽处理(在7 psi压力下处理5分钟、10分钟和15分钟)。在所有处理中,将果汁在玻璃瓶中于65°C下巴氏杀菌25分钟,并在预先灭菌的玻璃瓶中低温(5°C)储存。随着储存时间的延长,果汁的°Brix值、总糖、pH值和总酚含量下降,而微生物数量、可滴定酸度和还原糖含量在储存期间显著增加。添加KMS、柠檬酸以及蒸汽处理通过抑制多酚氧化酶的活性,减少了果汁的褐变,并在储存期间保持了果汁的颜色,使酶活性从0.571单位/毫升降至0.1单位/毫升。在所选择的处理方式中,添加KMS(100和150 ppm)以及在7 psi压力下对甘蔗进行5分钟和10分钟蒸汽处理的甘蔗汁在理化性质、感官品质方面变化最小,且微生物生长受到限制。经巴氏杀菌的亚硫酸化处理被证明最有利于在冷藏条件下将甘蔗汁的保质期延长至90天。同样,经蒸汽处理的甘蔗汁(在7 psi压力下处理10分钟和15分钟)在冷藏且不添加任何防腐剂的情况下可有效保存30天。