College of Optometry, University of Houston, Houston, TX 77204, USA.
Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030, USA.
Int J Mol Sci. 2023 Jan 4;24(2):931. doi: 10.3390/ijms24020931.
High-fat/sucrose diet feeding in mice causes loss of corneal nerve function and impairs corneal wound healing. While changing to a diet with a low fat/sugar composition and enrichments in complex carbohydrates mitigates the reduction in nerve function, it remains to be determined if it has an effect on corneal wound healing. In this study, 6-week-old C57BL/6 male mice were fed either a normal diet or a high-fat/sucrose diet for 20 weeks. A third group (diet reversal) was placed on a high-fat/sucrose diet for 10 weeks followed by a normal diet for an additional 10 weeks. A central corneal epithelial abrasion wound was created, and wound closure was monitored. Neutrophil and platelet recruitment was assessed by immunofluorescence microscopy. Mice fed the high-fat/sucrose diet-only had greater adiposity (p < 0.005) than normal diet-only fed mice; diet reversal markedly reduced adiposity. Following corneal abrasion, wound closure was delayed by ~6 h (p ≤ 0.01) and, at 30 h post-wounding, fewer neutrophils reached the wound center and fewer extravascular platelets were present at the limbus (p < 0.05). Diet restored normal wound closure and neutrophil and platelet influx in the injured cornea. These data suggest compositional changes to the diet may be an effective diet-based therapeutic strategy for maintaining or restoring corneal health.
高脂肪/蔗糖饮食喂养会导致角膜神经功能丧失，并损害角膜伤口愈合。虽然改为低脂肪/低糖成分的饮食，并增加复杂碳水化合物的摄入可以减轻神经功能的降低，但仍需要确定其对角膜伤口愈合是否有影响。在这项研究中，6 周龄的 C57BL/6 雄性小鼠被喂食正常饮食或高脂肪/蔗糖饮食 20 周。第三组（饮食逆转）先喂食高脂肪/蔗糖饮食 10 周，然后再喂食正常饮食 10 周。在中央角膜上皮擦伤后，监测伤口闭合情况。通过免疫荧光显微镜评估中性粒细胞和血小板的募集情况。仅喂食高脂肪/蔗糖饮食的小鼠比仅喂食正常饮食的小鼠有更大的肥胖程度（p<0.005）；饮食逆转显著降低了肥胖程度。角膜擦伤后，伤口闭合延迟约 6 小时（p≤0.01），在创伤后 30 小时，更少的中性粒细胞到达伤口中心，更少的血管外血小板存在于角膜缘（p<0.05）。饮食恢复了正常的伤口闭合以及中性粒细胞和血小板在受伤角膜中的浸润。这些数据表明，饮食成分的改变可能是维持或恢复角膜健康的有效饮食治疗策略。
