Central R & D Center, Medical & Bio Decision (MBD) Co., Ltd., Suwon-si 16229, Gyeonggi-do, Republic of Korea.
Department of Biomedical Engineering, Gachon University, Seongnam-si 13120, Gyeonggi-do, Republic of Korea.
Int J Mol Sci. 2023 Jan 5;24(2):1006. doi: 10.3390/ijms24021006.
Three-dimensional (3D) culture platforms have been adopted in a high-throughput screening (HTS) system to mimic in vivo physiological microenvironments. The automated dispenser has been established commercially to enable spotting or distributing non-viscous or viscous biomaterials onto microplates. However, there are still challenges to the precise and accurate dispensation of cells embedded in hydrogels such as Alginate- and Matrigel-extracellular matrices. We developed and improved an automated contact-free dispensing machine, the ASFA SPOTTER (V5 and V6), which is compatible with 96- and 384-pillar/well plates and 330- and 532-micropillar/well chips for the support of 3D spheroid/organoid models using bioprinting techniques. This enables the distribution of non-viscous and viscous biosamples, including chemical drugs and cancer cells, for large-scale drug screening at high speed and small volumes (20 to 4000 nanoliters) with no damage to cells. The ASFA SPOTTER (V5 and V6) utilizes a contact-free method that minimizes cross-contamination for the dispensation of encapsulated tissue cells with highly viscous scaffolds (over 70%). In particular, the SPOTTER V6 does not require a washing process and offers the advantage of almost no dead volume (defined as additional required sample volume, including a pre-shot and flushing shot for dispensing). It can be successfully applied for the achievement of an organoid culture in automation, with rapid and easy operation, as well as miniaturization for high-throughput screening. In this study, we report the advantages of the ASFA SPOTTER, which distributes standard-sized cell spots with hydrogels onto a 384-pillar/well plate with a fast dispensing speed, small-scale volume, accuracy, and precision.
三维（3D）培养平台已被应用于高通量筛选（HTS）系统，以模拟体内生理微环境。自动化分配器已商业化建立，能够将非粘性或粘性生物材料点样或分配到微孔板上。然而，对于嵌入水凝胶（如藻酸盐和基质胶）中的细胞的精确和准确分配仍然存在挑战。我们开发并改进了一种自动化无接触分配机，即 ASFA SPOTTER（V5 和 V6），它与 96 孔和 384 孔/孔板以及 330 孔和 532 微柱/孔芯片兼容，用于支持使用生物打印技术的 3D 球体/类器官模型。这使得非粘性和粘性生物样品（包括化学药物和癌细胞）的分配成为可能，用于高速和小体积（20 至 4000 纳升）的大规模药物筛选，且不会对细胞造成损伤。ASFA SPOTTER（V5 和 V6）利用无接触方法，最大限度地减少了对高粘性支架（超过 70%）包封组织细胞的分配中的交叉污染。特别是，SPOTTER V6 不需要清洗过程，并且具有几乎没有死体积（定义为分配所需的额外样品体积，包括预喷和冲洗喷）的优点。它可以成功地应用于自动化实现类器官培养，具有快速和易于操作的优点，并且适用于高通量筛选的小型化。在本研究中，我们报告了 ASFA SPOTTER 的优势，它可以以快速的分配速度、小体积、准确性和精密度将带有水凝胶的标准尺寸细胞点样分配到 384 孔/孔板上。
