Centre of Oxygen Research and Development (CORD), University of Liege, 4000 Liege, Belgium.
Research Unit FARAH, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Int J Mol Sci. 2023 Jan 5;24(2):1030. doi: 10.3390/ijms24021030.
We investigated the antioxidant potential of equine mesenchymal stem cells derived from muscle microbiopsies (mdMSCs), loaded by a water-soluble curcumin lysinate incorporated into hydroxypropyl-β-cyclodextrin (NDS27). The cell loading was rapid and dependent on NDS27 dosage (14, 7, 3.5 and 1 µM). The immunomodulatory capacity of loaded mdMSCs was evaluated by ROS production, on active and total myeloperoxidase (MPO) degranulation and neutrophil extracellular trap (NET) formation after neutrophil stimulation. The intracellular protection of loaded cells was tested by an oxidative stress induced by cumene hydroperoxide. Results showed that 10 min of mdMSC loading with NDS27 did not affect their viability while reducing their metabolism. NDS27 loaded cells in presence of 14, 7 µM NDS27 inhibited more intensively the ROS production, the activity of the MPO released and bound to the NET after neutrophil stimulation. Furthermore, loaded cells powerfully inhibited intracellular ROS production induced by cumene as compared to control cells or cyclodextrin-loaded cells. Our results showed that the loading of mdMSCs with NDS27 significantly improved their antioxidant potential against the oxidative burst of neutrophil and protected them against intracellular ROS production. The improved antioxidant protective capacity of loaded mdMSCs could be applied to target inflammatory foci involving neutrophils.
我们研究了通过水溶性姜黄素赖氨酸盐负载到羟丙基-β-环糊精（NDS27）中负载的来自肌肉活组织检查的马间充质干细胞（mdMSCs）的抗氧化潜力。细胞加载快速且依赖于 NDS27 剂量（14、7、3.5 和 1µM）。通过在中性粒细胞刺激后评估活性和总髓过氧化物酶（MPO）脱颗粒和中性粒细胞胞外陷阱（NET）形成的 ROS 产生来评估负载 mdMSCs 的免疫调节能力。通过 cumene 氢过氧化物诱导的氧化应激测试负载细胞的细胞内保护。结果表明，10 分钟的 NDS27 负载 mdMSC 不会影响其活力，同时降低其代谢。在存在 14、7µM NDS27 的情况下负载的细胞更强烈地抑制 ROS 产生、中性粒细胞刺激后释放和结合到 NET 的 MPO 活性。此外，与对照细胞或环糊精负载细胞相比，负载细胞可有力地抑制细胞内 cumene 诱导的 ROS 产生。我们的结果表明，用 NDS27 负载 mdMSCs 可显著提高其抗氧化能力，以抵抗中性粒细胞的氧化爆发，并保护它们免受细胞内 ROS 产生的影响。负载的 mdMSCs 的抗氧化保护能力的提高可应用于涉及中性粒细胞的炎症焦点。
