State Key Laboratory for Conservation and Utilization of Subtropical Agro-Biological Resources & Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, China.
Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USA.
Int J Mol Sci. 2023 Jan 6;24(2):1160. doi: 10.3390/ijms24021160.
The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a 'pollen- and seed-specific Gene Deletor' system that automatically excised all transgenes from the pollen and seeds of greenhouse-grown transgenic . In this study, we conducted seven field experiments over three consecutive years to evaluate the stability of transgene excision under field conditions. Our results showed that transgenes were stably excised from transgenic under field conditions with 100% efficiency. The stability of transgene excision was confirmed based on PCR, as well as the GUS staining patterns of various organs (roots, leaves, petiole, stem, flower, fruit, and seeds) from transgenic . In six transgenic lines (D4, D10, D31, D56, and D43), the transgenes were stably deleted in the T0 and T1 generations. Thus, the 'Gene Deletor' system is an efficient and reliable method to reduce pollen- and seed-mediated unintentional gene flow. This system might help to alleviate the food safety concerns associated with transgenic crops.
商业应用的转基因植物已严重受阻公众关注的潜在风险所构成的这种植物的生态系统和人类健康。以前，我们已经开发出了一种“花粉和种子特异性基因 Deletor”系统，自动切除所有的转基因花粉和种子的温室种植的转基因。在这项研究中，我们进行了七个田间试验连续三年评估基因切除的稳定性在田间条件下。我们的结果表明，转基因基因切除稳定在田间条件下，效率为 100%。的稳定性基因切除被证实基于 PCR，以及 GUS 染色模式的各种器官（根、叶、叶柄、茎、花、果实和种子）从转基因。在六个转基因株系（D4、D10、D31、D56 和 D43），该基因被稳定删除的 T0 和 T1 代。因此，“基因 Deletor”系统是一种有效的和可靠的方法来减少花粉和种子介导的无意基因流。该系统可能有助于减轻与转基因作物相关的食品安全问题。
