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- 和 - 白藜芦醇对 ANO1 的抑制作用及其对人前列腺癌细胞 PC-3 的抗癌活性。

Inhibition of ANO1 by - and -Resveratrol and Their Anticancer Activity in Human Prostate Cancer PC-3 Cells.

机构信息

College of Pharmacy and Yonsei, Institute of Pharmaceutical Sciences, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon 21983, Republic of Korea.

Department of Physiology, College of Medicine, Dongguk University, 123 Dongdae-ro, Gyeongju 38066, Republic of Korea.

出版信息

Int J Mol Sci. 2023 Jan 7;24(2):1186. doi: 10.3390/ijms24021186.

DOI:10.3390/ijms24021186
PMID:36674697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9862168/
Abstract

Anoctamin1 (ANO1), a calcium-activated chloride channel, is involved in the proliferation, migration, and invasion of various cancer cells including head and neck squamous cell carcinoma, lung cancer, and prostate cancer. Inhibition of ANO1 activity or downregulation of ANO1 expression in these cancer cells is known to exhibit anticancer effects. Resveratrol, a natural polyphenol abundant in wines, grapes, berries, soybeans, and peanuts, shows a wide variety of biological effects including anti-inflammatory, antioxidant, and anticancer activities. In this study, we investigated the effects of two stereoisomers of resveratrol on ANO1 activity and found that - and -resveratrol inhibited ANO1 activity with different potencies. - and -resveratrol inhibited ANO1 channel activity with IC values of 10.6 and 102 μM, respectively, and had no significant effect on intracellular calcium signaling at 10 and 100 μM, respectively. In addition, -resveratrol downregulated mRNA and protein expression levels of ANO1 more potently than -resveratrol in PC-3 prostate cancer cells. - and -resveratrol significantly reduced cell proliferation and cell migration in an ANO1-dependent manner, and both resveratrol isomers strongly increased caspase-3 activity, PARP cleavage, and apoptotic sub-G1 phase ratio in PC-3 cells. These results revealed that -resveratrol is a potent inhibitor of ANO1 and exhibits ANO1-dependent anticancer activity against human metastatic prostate cancer PC-3 cells.

摘要

Anoctamin1（ANO1），一种钙激活氯离子通道，参与多种癌细胞的增殖、迁移和侵袭，包括头颈部鳞状细胞癌、肺癌和前列腺癌。已知抑制这些癌细胞中的 ANO1 活性或下调 ANO1 表达会产生抗癌作用。白藜芦醇是一种天然多酚，广泛存在于葡萄酒、葡萄、浆果、大豆和花生中，具有多种生物学作用，包括抗炎、抗氧化和抗癌活性。在这项研究中，我们研究了两种白藜芦醇立体异构体对 ANO1 活性的影响，发现-和-白藜芦醇以不同的效力抑制 ANO1 活性。-和-白藜芦醇对 ANO1 通道活性的抑制 IC 值分别为 10.6 和 102 μM，在 10 和 100 μM 时分别对细胞内钙信号无显著影响。此外，-白藜芦醇在 PC-3 前列腺癌细胞中下调 ANO1 的 mRNA 和蛋白表达水平比-白藜芦醇更有效。-和-白藜芦醇以 ANO1 依赖的方式显著降低细胞增殖和细胞迁移，两种白藜芦醇异构体均强烈增加 PC-3 细胞中的 caspase-3 活性、PARP 切割和凋亡亚 G1 期比例。这些结果表明，-白藜芦醇是 ANO1 的有效抑制剂，对人转移性前列腺癌 PC-3 细胞具有 ANO1 依赖性抗癌活性。

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3
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