Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
Department of Horticulture, Faculty of Agriculture, Minia University, El-Minia 61517, Egypt.
Int J Mol Sci. 2023 Jan 13;24(2):1560. doi: 10.3390/ijms24021560.
Drought stress is a severe environmental issue that threatens agriculture at a large scale. PHYTOCHROMES (PHYs) are important photoreceptors in plants that control plant growth and development and are involved in plant stress response. The aim of this study was to identify the role of in the tomato cv. 'Moneymaker' under drought conditions. The tomato genome contains five , among which mutant lines in tomato and ( and ) were used. Compared to the WT, and mutants exhibited drought tolerance and showed inhibition of electrolyte leakage and malondialdehyde accumulation, indicating decreased membrane damage in the leaves. Both mutants also inhibited oxidative damage by enhancing the expression of reactive oxygen species (ROS) scavenger genes, inhibiting hydrogen peroxide (HO) accumulation, and enhancing the percentage of antioxidant activities via DPPH test. Moreover, expression levels of several aquaporins were significantly higher in and , and the relative water content (RWC) in leaves was higher than the RWC in the WT under drought stress, suggesting the enhancement of hydration status in the mutants. Therefore, inhibition of oxidative damage in and mutants may mitigate the harmful effects of drought by preventing membrane damage and conserving the plant hydrostatus.
干旱胁迫是一个严重的环境问题，它大规模地威胁着农业。光敏色素（PHYs）是植物中重要的光受体，它们控制着植物的生长和发育，并参与植物的应激反应。本研究旨在鉴定 在番茄 cv. 'Moneymaker' 中的作用。番茄基因组包含五个 ，其中番茄 和 （ 和 ）的突变体被用于研究。与 WT 相比， 和 突变体表现出耐旱性，并表现出抑制电解质渗漏和丙二醛积累的特性，表明叶片的膜损伤减少。两个 突变体还通过增强活性氧 (ROS) 清除基因的表达、抑制过氧化氢 (HO) 积累以及通过 DPPH 测试增强抗氧化活性的百分比，抑制氧化损伤。此外，在 和 中几个水通道蛋白的表达水平显著升高，并且在干旱胁迫下叶片的相对含水量 (RWC) 高于 WT 的 RWC，表明 突变体的水合状态得到增强。因此， 通过防止膜损伤和维持植物水合状态， 和 突变体中氧化损伤的抑制可能减轻干旱的有害影响。
