Yang Jingli, Wang Hanzeng, Zhao Shicheng, Liu Xiao, Zhang Xin, Wu Weilin, Li Chenghao
State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin, China.
School of Pharmacy, Harbin University of Commerce, Harbin, China.
Front Plant Sci. 2020 Nov 11;11:528550. doi: 10.3389/fpls.2020.528550. eCollection 2020.
The application of drought stress-regulating transcription factors (TFs) offers a credible way to improve drought tolerance in plants. However, many drought resistant TFs always showed unintended adverse effects on plant growth or other traits. Few studies have been conducted in trees to evaluate and overcome the pleiotropic effects of drought tolerance TFs. Here, we report the dose-dependent effect of the gene on its overexpression in . High- and moderate-level overexpression of significantly increased drought tolerance in a dose-dependent manner. However, the OE18 plants showed stunted growth under normal conditions, but they were also more sensitive to infection than wild type (WT) and OE14 plants. While, OE14 showed normal growth, the pathogen tolerance of them was not significantly different from WT. Many stress-responsive genes were up-regulated in OE18 and OE14 compared to WT, especially for OE18 plants. Meanwhile, more pathogen tolerance related genes were down-regulated in OE18 compared to OE14 and WT plants. We achieved improved drought tolerance by adjusting the increased levels of exogenous genes to avoid the occurrence of growth reduction and reduced disease tolerance.
应用干旱胁迫调节转录因子（TFs）为提高植物耐旱性提供了一种可靠的方法。然而，许多抗旱转录因子总是对植物生长或其他性状产生意想不到的不利影响。在树木中，很少有研究评估和克服耐旱转录因子的多效性影响。在此，我们报道了该基因在其过表达时的剂量依赖性效应。该基因的高水平和中等水平过表达以剂量依赖方式显著提高了耐旱性。然而，OE18植株在正常条件下生长受阻，但它们也比野生型（WT）和OE14植株对感染更敏感。而OE14生长正常，其对病原体的耐受性与WT无显著差异。与WT相比，OE18和OE14中许多胁迫响应基因上调，尤其是OE18植株。同时，与OE14和WT植株相比，OE18中更多与病原体耐受性相关的基因下调。我们通过调整外源基因的增加水平来提高耐旱性，以避免生长降低和抗病性降低的发生。
