Esfahanian Vahid, Ejeian Fatemeh, Mohebinia Hajar, Zojaji Nejad Zahra Sadat, Yazdchi Maryam, Ebrahimi Dastgerdi Maziar, Ebrahimi Dastgerdi Mehrnoush, Nasr-Esfahani Mohammad Hossein
Department of Periodontic, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan 8155139998, Iran.
Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan 8159358686, Iran.
Life (Basel). 2022 Dec 28;13(1):89. doi: 10.3390/life13010089.
Periodontal regeneration through the employment of bone substitutes has become a feasible strategy in animal and clinical studies. In this regard, we aimed to compare the periodontal ligament stem cell behavior in the vicinity of various bone grafting substitutes. Three types of popular bone substitutes, including allografts (Regen), xenografts (Cerabone), and alloplasts (Osteon) were studied in this experimental survey. The cellular attachment was assessed after four hours using the MTS assay and SEM imaging. In addition, cellular proliferation was investigated after 1, 3, 5, and 7 days through MTS assay. Osteogenesis was studied after 21 days of cell culture in a differentiation medium (DM+) and a normal medium (DM-), by employing real-time PCR and alizarin red staining. The highest cellular attachment was seen in the xenograft group with a significant difference in comparison to the other grafting materials. Despite the relatively low primary attachment of cells to allografts, the allograft group showed the highest total proliferation rate, while the lowest proliferation capacity was found in the alloplast group. Osteogenesis fount to be accelerated mostly by xenografts in both mediums (DM+ and DM-) after 3 weeks, while alloplasts showed the lowest osteogenesis. This study revealed that the type of bone substitutes used in regenerative treatments can affect cellular behavior and as a whole allografts and xenografts showed better results.
在动物和临床研究中，通过使用骨替代物实现牙周再生已成为一种可行的策略。在这方面，我们旨在比较不同骨移植替代物附近的牙周膜干细胞行为。在本实验研究中，对三种常见的骨替代物进行了研究，包括同种异体移植物（Regen）、异种移植物（Cerabone）和人工合成材料（Osteon）。使用MTS法和扫描电子显微镜成像在4小时后评估细胞附着情况。此外，通过MTS法在1、3、5和7天后研究细胞增殖情况。在细胞于分化培养基（DM +）和正常培养基（DM -）中培养21天后，通过实时聚合酶链反应和茜素红染色研究成骨情况。异种移植物组的细胞附着率最高，与其他移植材料相比有显著差异。尽管细胞对同种异体移植物的初始附着相对较低，但同种异体移植物组的总增殖率最高，而人工合成材料组的增殖能力最低。3周后，在两种培养基（DM +和DM -）中，异种移植物大多能加速成骨，而人工合成材料的成骨能力最低。这项研究表明，再生治疗中使用的骨替代物类型会影响细胞行为，总体而言，同种异体移植物和异种移植物显示出更好的效果。
