Zuo Meng-Ting, Gong Meng-Die, Ma Xiao, Xu Wen-Bo, Wang Zi-Yuan, Tang Mo-Huan, Wu Yong, Liu Zhao-Ying
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China.
Metabolites. 2022 Dec 24;13(1):33. doi: 10.3390/metabo13010033.
Asian () has a wide range of pharmacological activities. However, its strong toxicity limits its potential development and application. Interestingly, there are significant gender differences in toxicity in rats. This work aimed to elucidate the overall absorption, distribution, metabolism, and excretion (ADME) of whole crude extract in female and male rats using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/QqTOF-MS), which facilitates determining the reasons for the gender differences in toxicity. A total of 25 absorbed bioactive components and 3 related produced metabolites were tentatively identified in female rats, while only 17 absorbed bioactive components and 3 related produced metabolites were identified in male rats. By comparison of peak intensities, most compounds were found to be more active in absorption, distribution and excretion in female rats than in male rats, which showed that female rats were more sensitive to . This study was the first to investigate the multicomponent in vivo process of in rats and compare the differences between sexes. It was hypothesized that differences in the absorption of gelsedine-type alkaloids were one of the main reasons for the sex differences in toxicity.
亚洲（某种物质）具有广泛的药理活性。然而，其强毒性限制了其潜在的开发和应用。有趣的是，在大鼠中该物质的毒性存在显著的性别差异。这项工作旨在使用高效液相色谱-四极杆飞行时间质谱联用技术（HPLC/QqTOF-MS）阐明雌性和雄性大鼠中整个（该物质）粗提取物的整体吸收、分布、代谢和排泄（ADME）情况，这有助于确定毒性性别差异的原因。在雌性大鼠中初步鉴定出总共25种吸收的生物活性成分和3种相关产生的代谢物，而在雄性大鼠中仅鉴定出17种吸收的生物活性成分和3种相关产生的代谢物。通过峰强度比较，发现大多数化合物在雌性大鼠中的吸收、分布和排泄比在雄性大鼠中更活跃，这表明雌性大鼠对（该物质）更敏感。本研究首次研究了（该物质）在大鼠体内的多成分过程并比较了性别差异。据推测，格赛丁型生物碱吸收的差异是（该物质）毒性性别差异的主要原因之一。
