Suda Y, Suzuki M, Ikawa Y, Aizawa S
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
J Cell Physiol. 1987 Oct;133(1):197-201. doi: 10.1002/jcp.1041330127.
The proliferative potential of embryonic stem cells was examined. In contrast to the current concept of the finite life-span being the hallmark of normal cells, we have been able to maintain these embryonic stem cells in vitro up to about 250 cumulative doublings with no indication of "crisis" or transformation. These cells could be considered normal on the basis of: (1) their apparently normal diploid karyotype, (2) their ability to extensively colonize embryos without causing tumors and developmental anomalies, and (3) their ability to form normal gametes when differentiated into the germ-line. These results suggest that embryonic stem cells prior to differentiation into germ and somatic cells are indeed immortal.
对胚胎干细胞的增殖潜能进行了检测。与当前认为有限寿命是正常细胞标志的概念相反，我们已能够在体外将这些胚胎干细胞维持至约250次累积倍增，且没有“危机”或转化的迹象。基于以下几点，这些细胞可被视为正常：（1）它们明显正常的二倍体核型；（2）它们广泛定殖于胚胎而不引起肿瘤和发育异常的能力；（3）它们在分化为生殖系时形成正常配子的能力。这些结果表明，在分化为生殖细胞和体细胞之前的胚胎干细胞确实是永生的。
