Sanchez-Gonzalez Ma Esther, Mora-Herrera Martha E, Wong-Villarreal Arnoldo, De La Portilla-López Nadia, Sanchez-Paz Laura, Lugo Jorge, Vaca-Paulín Rocio, Del Aguila Pedro, Yañez-Ocampo Gustavo
Laboratory of Edaphology and Environment, Faculty of Sciences, Autonomous University of the State of Mexico, Toluca 50000, Mexico.
Tenancingo Universitary Center, Autonomous University of the State of Mexico, Toluca 50000, Mexico.
Microorganisms. 2022 Dec 23;11(1):49. doi: 10.3390/microorganisms11010049.
Phosphate-solubilizing bacteria (PSB) transform precipitated inorganic phosphorus into soluble orthophosphates. This study evaluated the efficiency of tricalcium and iron phosphate solubilization in Pikovskaya medium using five bacterial strains (A1, A2, A3, A5, and A6) cultured in acidic and alkaline pH levels. The bacterial strain that proved to be more efficient for P solubilization and was tolerant to pH variations was selected for assessing bacterial growth and P solubilization with glucose and sucrose in the culture medium. The bacterial strains were identified through 16S rRNA gene sequencing as A1, (A2), (A3), (A5), and (A6). These five bacterial strains grew, tolerated pH changes, and solubilized inorganic phosphorus. The bacterial strain A3 solubilized FePO (4 mg L) and Ca(PO) (50 mg L). P solubilization was assayed with glucose and sucrose as carbon sources for A3 ( MN100586). After four culture days, Ca(PO) was solubilized, reaching 246 mg L with sucrose in culture media. Using glucose as a carbon source, FePO was solubilized and reached 282 mg L in six culture days. Our findings were: , and as new bacteria, can be reported as P solubilizers with tolerance to acidic or alkaline pH levels. The bacterial strain grew using two sources of inorganic phosphorus and carbon, and it tolerated pH changes. For that reason, it is an ideal candidate for inorganic phosphorus solubilization and future production as a biofertilizer.
解磷细菌（PSB）将沉淀的无机磷转化为可溶性正磷酸盐。本研究在酸性和碱性pH水平下培养5种细菌菌株（A1、A2、A3、A5和A6），评估了它们在皮氏培养基中溶解磷酸三钙和磷酸铁的效率。选择了对磷溶解更有效且耐pH变化的细菌菌株，以评估其在培养基中利用葡萄糖和蔗糖的生长及磷溶解情况。通过16S rRNA基因测序将这些细菌菌株鉴定为A1、（A2）、（A3）、（A5）和（A6）。这5种细菌菌株能够生长、耐受pH变化并溶解无机磷。细菌菌株A3溶解了FePO₄（4 mg/L）和Ca₃(PO₄)₂（50 mg/L）。以葡萄糖和蔗糖作为A3（MN100586）的碳源测定磷溶解情况。培养4天后，Ca₃(PO₄)₂被溶解，在培养基中添加蔗糖时达到246 mg/L。以葡萄糖作为碳源时，FePO₄被溶解，在6天培养后达到282 mg/L。我们的研究结果是：作为新的细菌，A1、A2和A6可被报告为耐酸性或碱性pH水平的解磷菌。该细菌菌株利用两种无机磷和碳源生长，并且耐受pH变化。因此，它是无机磷溶解及未来作为生物肥料生产的理想候选菌株。
