College of Life Science and Agroforestry, Qiqihar University, Qiqihar 161006, China.
Heilongjiang Provincial Technology Innovation center of Agromicrobial Preparation Industrialization, Qiqihar 161006, China.
Mol Plant Microbe Interact. 2020 Oct;33(10):1222-1231. doi: 10.1094/MPMI-04-20-0106-R. Epub 2020 Aug 11.
Elucidation of the underlying mechanisms of plant growth promotion of rhizobacteria is very important. This study explored the mechanism by which LZP02 promotes growth in rice roots through proteomic, transcriptomic, and metabolomic techniques. The results showed that LZP02 promoted the absorption of phosphorous, calcium, and magnesium ions by colonization of rice roots and enhanced peroxidase, catalase, superoxide dismutase, and CaMg adenosine triphosphatase activities and chlorophyll contents in rice. The proteomic results showed that most of the differentially expressed proteins were involved in carbohydrate metabolism and that the biosynthesis of other secondary metabolites was also increased. According to RNA-seq and reverse transcription-quantitative PCR analyses, expression of some genes involved in carbohydrate metabolism and phenylpropanoid biosynthesis was upregulated in rice roots. Regarding metabolomics, phenylpropanoid biosynthesis, starch and sucrose metabolism, the pentose phosphate pathway, and glyoxylate and dicarboxylate metabolism were increased. The results indicated that LZP02 promoted the growth of rice roots by enhancing carbohydrate metabolism and phenylpropanoid biosynthesis.
阐明根际促生菌促进植物生长的内在机制非常重要。本研究通过蛋白质组学、转录组学和代谢组学技术探索了LZP02 促进水稻根系生长的机制。结果表明，LZP02 通过定殖水稻根促进磷、钙和镁离子的吸收，并增强水稻过氧化物酶、过氧化氢酶、超氧化物歧化酶和 CaMg 三磷酸腺苷酶的活性和叶绿素含量。蛋白质组学结果表明，大多数差异表达蛋白参与碳水化合物代谢，其他次生代谢物的生物合成也增加。根据 RNA-seq 和反转录定量 PCR 分析，水稻根系中一些参与碳水化合物代谢和苯丙烷生物合成的基因表达上调。在代谢组学方面，苯丙烷生物合成、淀粉和蔗糖代谢、戊糖磷酸途径以及乙醛酸和二羧酸代谢增加。结果表明，LZP02 通过增强碳水化合物代谢和苯丙烷生物合成促进水稻根系生长。
