Sen Arup, Saha Niharendu, Sarkar Arindam, Poddar Ratneswar, Pramanik Krishnendu, Samanta Anwesha
Department of Agricultural Chemistry and Soil Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia India.
Regional Research Station (R and L Zone), Bidhan Chandra Krishi Viswavidyalaya, Kadamkanan, Jhargram India.
3 Biotech. 2024 Sep;14(9):197. doi: 10.1007/s13205-024-04042-2. Epub 2024 Aug 8.
Phosphorus (P) is the key to several structural molecules and catalyzes numerous biochemical reactions in plant body besides its involvement in energy transfer. Any deficit in P availability is likely to result in reduced RNA and protein content, inhibiting crop growth and development. Thus, availability of soil P is extremely crucial for plant growth especially in acid soils of India, where most of the fraction is bound to solid phase rendering their availability. The present communication deals with the isolation of elite phosphate-solubilizing bacterial (PSB) strains from the acid soils to work out their ability to improve the fertilizer P use efficiency in the acidic environment. Initially twenty-six bacteria were isolated from the acid soils of Northeastern India. Among them, ten bacteria were selected based on formation of halo zone in the Pikovskaya agar plate. In addition, these bacteria were able to solubilize insoluble zinc (Zn) and potassium (K). The isolates were subject to in vitro optimization for P solubilization under different insoluble P source utilization and at different pH and salinity conditions. Strains AN3, AN11, and AN21 exhibited significant solubilization of insoluble P, Zn, and K, and were identified as sp., sp., and . These three bacteria solubilized 206.53 to 254.08 µg mL P, 79.7 to 177.55 µg mL Zn, and 0.96 to 1.56 µg mL K from insoluble minerals. Their performance was further evaluated in pot culture experiment using green gram as test crop. These three bacteria were found to improve P uptake and dry matter accumulation in green gram plant substantially. Seed bio-priming with the PSB strains enhanced the efficiency of added P fertilizer, resulting in a 1.40 to 1.52 times higher effectiveness compared to the control. On the whole, AN11 may be ranked as best inoculant for the acidic soils of Northeastern India.
The online version contains supplementary material available at 10.1007/s13205-024-04042-2.
磷（P）是多种结构分子的关键成分，除了参与能量转移外，还催化植物体内众多生化反应。磷供应不足可能会导致RNA和蛋白质含量降低，从而抑制作物生长发育。因此，土壤磷的有效性对植物生长极为关键，尤其是在印度的酸性土壤中，大部分磷以固相形式存在，难以被植物利用。本通讯报道了从酸性土壤中分离优良解磷细菌（PSB）菌株的研究，以确定它们在酸性环境中提高肥料磷利用效率的能力。最初从印度东北部的酸性土壤中分离出26株细菌。其中，根据在皮科夫斯卡娅琼脂平板上形成的晕圈，挑选出10株细菌。此外，这些细菌还能够溶解不溶性锌（Zn）和钾（K）。在不同的不溶性磷源利用以及不同的pH和盐度条件下，对这些分离菌株进行了体外解磷优化。菌株AN3、AN11和AN21表现出对不溶性磷、锌和钾的显著溶解能力，分别鉴定为 属、 属和 属。这三种细菌从不溶性矿物质中溶解出206.53至254.08μg/mL的磷、79.7至177.55μg/mL的锌和0.96至1.56μg/mL的钾。使用绿豆作为试验作物，通过盆栽试验进一步评估了它们的性能。发现这三种细菌能显著提高绿豆植株对磷的吸收和干物质积累。用PSB菌株进行种子生物引发提高了添加磷肥的效率，与对照相比，有效性提高了1.40至1.52倍。总体而言，AN11可能是印度东北部酸性土壤的最佳接种剂。
在线版本包含可在10.1007/s13205-024-04042-（此处原文有误，应为04042-2）2获取的补充材料。
