Tian Kejian, Yu Yue, Qiu Qing, Sun Xuejian, Meng Fanxing, Bi Yuanping, Gu Jinming, Wang Yibing, Zhang Fenglin, Huo Hongliang
School of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun 130117, China.
Jilin Province Water Resources and Hydropower Consultative Company of P.R. China, Changchun 130021, China.
Microorganisms. 2022 Dec 26;11(1):67. doi: 10.3390/microorganisms11010067.
Bisphenol A (BPA) pollution poses an increasingly serious problem. BPA has been detected in a variety of environmental media and human tissues. Microbial degradation is an effective method of environmental BPA remediation. However, BPA is also biotoxic to microorganisms. In this study, DSSKP-R-001 (R-001) was used to degrade BPA, and the effects of BPA on the growth metabolism, gene expression patterns, and toxicity-resistance mechanisms of were analyzed. The results showed that R-001 degraded 51.2% of 5 mg/L BPA and that 40 mg/L BPA was the maximum BPA concentration tolerated by strain R-001. Cytochrome P450 monooxygenase and multicopper oxidases played key roles in BPA degradation. However, BPA was toxic to strain R-001, exhibiting nonlinear inhibitory effects on the growth and metabolism of this bacterium. R-001 bacterial biomass, total protein content, and ATP content exhibited V-shaped trends as BPA concentration increased. The toxic effects of BPA included the downregulation of R-001 genes related to glycolysis/gluconeogenesis, pentose phosphate metabolism, and glyoxylate and dicarboxylate metabolism. Genes involved in aspects of the BPA-resistance response, such as base excision repair, osmoprotectant transport, iron-complex transport, and some energy metabolisms, were upregulated to mitigate the loss of energy associated with BPA exposure. This study helped to clarify the bacterial mechanisms involved in BPA biodegradation and toxicity resistance, and our results provide a theoretical basis for the application of strain R-001 in BPA pollution treatments.
双酚A（BPA）污染问题日益严重。在多种环境介质和人体组织中均检测到了BPA。微生物降解是环境中BPA修复的有效方法。然而，BPA对微生物也具有生物毒性。本研究采用DSSKP-R-001（R-001）降解BPA，并分析了BPA对其生长代谢、基因表达模式及抗毒机制的影响。结果表明，R-001可降解5 mg/L BPA中的51.2%，40 mg/L BPA是菌株R-001所能耐受的最大BPA浓度。细胞色素P450单加氧酶和多铜氧化酶在BPA降解中起关键作用。然而，BPA对菌株R-001有毒性，对该细菌的生长和代谢表现出非线性抑制作用。随着BPA浓度的增加，R-001的细菌生物量、总蛋白含量和ATP含量呈V形趋势。BPA的毒性作用包括下调R-001中与糖酵解/糖异生、磷酸戊糖代谢以及乙醛酸和二羧酸代谢相关的基因。参与BPA抗性反应的基因，如碱基切除修复、渗透保护剂转运、铁复合物转运以及一些能量代谢相关基因被上调，以减轻与BPA暴露相关的能量损失。本研究有助于阐明细菌参与BPA生物降解和抗毒的机制，研究结果为菌株R-001在BPA污染处理中的应用提供了理论依据。
