Park Young Kyoung, Chin Young-Wook
Research Group of Traditional Food, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of Korea.
Microorganisms. 2023 Aug 22;11(9):2132. doi: 10.3390/microorganisms11092132.
Bisphenol A (BPA), one of the most widely used plasticizers, is an endocrine-disrupting chemical that is released from plastic products. The aim of this study was to screen and characterize bacteria with excellent BPA-degrading abilities for application in foods. BPA degradation ability was confirmed in 127 of 129 bacterial strains that were isolated from fermented soybean foods. Among the strains, P74, which showed the highest BPA degradation performance, degraded 97.2% of 10 mg/L of BPA within 9 h. This strain not only showed a fairly stable degradation performance (min > 88.2%) over a wide range of temperatures (30-45 °C) and pH (5.0-9.0) but also exhibited a degradation of 63% against high concentrations of BPA (80 mg/L). The metabolites generated during the degradation were analyzed using high-performance liquid chromatography-mass spectrometry, and predicted degradation pathways are tentatively proposed. Finally, the application of this strain to soybean fermentation was conducted to confirm its applicability in food.
双酚A（BPA）是使用最广泛的增塑剂之一，是一种可从塑料制品中释放出来的内分泌干扰化学物质。本研究的目的是筛选并鉴定具有出色双酚A降解能力的细菌，以便应用于食品领域。从发酵豆制品中分离出的129株细菌中，有127株证实具有双酚A降解能力。其中，双酚A降解性能最高的P74菌株在9小时内降解了10 mg/L双酚A的97.2%。该菌株不仅在较宽的温度范围（30-45°C）和pH范围（5.0-9.0）内表现出相当稳定的降解性能（最低>88.2%），而且对高浓度双酚A（80 mg/L）也有63%的降解率。利用高效液相色谱-质谱联用仪分析了降解过程中产生的代谢产物，并初步推测了降解途径。最后，将该菌株应用于大豆发酵，以确认其在食品中的适用性。
