National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing, 100124, PR China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing, 100124, PR China.
Chemosphere. 2021 Oct;280:130707. doi: 10.1016/j.chemosphere.2021.130707. Epub 2021 May 4.
Wastewater containing 2,4,6-trichlorophenol (2,4,6-TCP) is highly toxic and causes harmful effects on aquatic ecosystems and human health. In this study, wastewater containing high levels of 2,4,6-TCP was successfully co-metabolized by introducing municipal domestic wastewater (MDW) as the co-catabolic carbon source. The concentration of degraded 2,4,6-TCP increased from 0 to 208.71 mg/L by adjusting the influent MDW volume during a 150-day-long operation. An MDW dose of 500 mL was found optimal, with an average concentration of 250 mgCOD/L. Unlike the long-term experiment, changing the MDW adding mode in a typical cycle further increased the concentration of 2,4,6-TCP removed to 317 mg/L. The main MDW components, such as the sugars, VFAs, and slowly biodegradable organic substances, improved 2,4,6-TCP degradation, achieving a TOC removal efficiency of 90.98% and a dechlorination efficiency of 100%. The MDW level did not change the 2,4,6-TCP degradation rate (μ) in a typical cycle compared to the single carbon source, and the μ remained at a high level of 50 mg 2,4,6-TCP/h. Macrogenetic analysis demonstrated that MDW addition promoted the growth of 43 bacterial genera (41.49%) responsible for 2,4,6-TCP degradation and intermediates' metabolism. The key genes for 2,4,6-TCP metabolism (pcpA, chqB, mal-r, pcaI, pcaF, and fadA) were detected in the activated sludge, which were distributed among the 43 genera. To conclude, this study proposes a new carbon source for co-metabolism to treat 2,4,6-TCP-polluted wastewater.
含 2,4,6-三氯苯酚（2,4,6-TCP）的废水毒性很高，对水生生态系统和人类健康造成有害影响。在本研究中，通过引入城市生活污水（MDW）作为共代谢碳源，成功地共代谢了高浓度的 2,4,6-TCP。在 150 天的运行过程中，通过调节进水 MDW 体积，使降解的 2,4,6-TCP 浓度从 0 增加到 208.71mg/L。发现 MDW 剂量为 500mL 是最佳的，平均浓度为 250mgCOD/L。与长期实验不同，在典型周期中改变 MDW 添加方式进一步将去除的 2,4,6-TCP 浓度提高到 317mg/L。MDW 的主要成分，如糖、VFAs 和缓慢生物降解的有机物质，提高了 2,4,6-TCP 的降解，TOC 去除效率达到 90.98%，脱氯效率达到 100%。与单一碳源相比，在典型周期中，MDW 水平并未改变 2,4,6-TCP 降解速率（μ），μ 仍保持在 50mg 2,4,6-TCP/h 的高水平。宏基因组分析表明，MDW 的添加促进了 43 个负责 2,4,6-TCP 降解和中间代谢的细菌属（41.49%）的生长。在活性污泥中检测到 2,4,6-TCP 代谢的关键基因（pcpA、chqB、mal-r、pcaI、pcaF 和 fadA），这些基因分布在 43 个属中。总之，本研究提出了一种新的共代谢碳源来处理 2,4,6-TCP 污染废水。
