Park Yeong-Jun, Kang Gi-Ung, Jeong Minsoo, Singh Vineet, Kim Jongho, Lee Kyunghyun, Choi Eun-Jin, Kim Heui-Jin, Lee Seungjun, Lee Sook-Young, Oem Jae-Ku, Shin Jae-Ho
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Animal and Plant Quarantine Agency, Kimcheon-si 39660, Republic of Korea.
Microorganisms. 2022 Dec 30;11(1):98. doi: 10.3390/microorganisms11010098.
Downer cow can be caused by muscular paralysis, neurological damage, metabolic disorder, and/or the complication of microbial infection. However, downer cow with unknown etiology is issued because of the non-detection of its bacterial etiological agent. In this study, differences in the bacterial community in brain tissues between downer cattle with unknown etiology and healthy slaughtered cattle are investigated. Bacterial diversity and representative genera between downer and normal cattle were significantly different (p < 0.05). There are significant differences in representative genera of downer and normal cattle, especially the significance, fold change, and area under the receiver operating characteristic curve score (p < 0.05). Furthermore, the prediction of functional genes in brain microbiota between the downer and normal cattle revealed differences in the cluster of orthologous gene categories, such as lipid transport and metabolism, secondary metabolite biosynthesis, and signal transduction (p < 0.05). This study revealed a significant difference in microbiota between the downer and normal cattle. Thus, we demonstrate that representative genera from downer cattle through 16S rRNA gene amplicon sequencing and microbiota analysis have the potential as candidates for bacterial etiological agents for downer cow.
卧地不起的母牛可能由肌肉麻痹、神经损伤、代谢紊乱和/或微生物感染并发症引起。然而，病因不明的卧地不起的母牛是由于未检测到其细菌病原体而出现的。在本研究中，调查了病因不明的卧地不起的母牛与健康屠宰牛脑组织中细菌群落的差异。卧地不起的母牛和正常牛之间的细菌多样性和代表性属存在显著差异（p < 0.05）。卧地不起的母牛和正常牛的代表性属存在显著差异，尤其是显著性、倍数变化和受试者工作特征曲线下面积得分（p < 0.05）。此外，卧地不起的母牛和正常牛脑微生物群中功能基因的预测显示，直系同源基因类别存在差异，如脂质转运和代谢、次级代谢产物生物合成和信号转导（p < 0.05）。本研究揭示了卧地不起的母牛和正常牛之间微生物群的显著差异。因此，我们证明，通过16S rRNA基因扩增子测序和微生物群分析，来自卧地不起的母牛的代表性属有潜力作为卧地不起的母牛细菌病原体的候选者。
