Ņečajeva Jevgenija, Borodušķe Anete, Nikolajeva Vizma, Seņkovs Māris, Kalniņa Ineta, Roga Ance, Skinderskis Edmunds, Fridmanis Dāvids
Institute of Plant Protection Research 'Agrihorts', Latvia University of Life Sciences and Technologies, Paula Lejiņa Iela 2, LV-3004 Jelgava, Latvia.
Microbial Strain Collection of Latvia, Faculty of Biology, University of Latvia, Jelgavas Iela 1, LV-1004 Rīga, Latvia.
Microorganisms. 2023 Jan 11;11(1):184. doi: 10.3390/microorganisms11010184.
Fungi colonizing the surface and endosphere of two widespread Poaceae weed species, Avena fatua and Echinochloa crus-galli, were isolated to compare the taxonomic composition between the plant species, location, and year of the seed collection. The seed-degrading potential of Fusarium isolated from the seeds was tested by inoculating seeds of E. crus-galli with spore suspension. Molecular identification of epiphytic and endophytic fungal genera was performed by sequencing the ITS region of rDNA. Endophytes comprised of significantly lower fungal richness compared to epiphytes. A significant taxonomic overlap was observed between the endosphere and seed surface. The most abundant genera were Alternaria, Fusarium, Cladosporium, and Sarocladium. Analysis of similarities and hierarchical clustering showed that microbial communities were more dissimilar between the two plant species than between the years. Fusarium isolates with a high potential to infect and degrade E. crus-galli seeds in laboratory conditions belong to F. sporotrichioides and F. culmorum.
对两种广泛分布的禾本科杂草物种——野燕麦和稗草的表面及内生真菌进行分离，以比较植物物种、种子采集地点和年份之间的分类组成。通过用孢子悬浮液接种稗草种子，测试了从种子中分离出的镰刀菌的种子降解潜力。通过对rDNA的ITS区域进行测序，对附生和内生真菌属进行了分子鉴定。与附生真菌相比，内生真菌的真菌丰富度明显较低。在内生菌和种子表面之间观察到明显的分类重叠。最丰富的属是链格孢属、镰刀菌属、枝孢属和帚枝霉属。相似性分析和层次聚类表明，两种植物物种之间的微生物群落差异大于年份之间的差异。在实验室条件下具有高感染和降解稗草种子潜力的镰刀菌分离株属于拟分枝孢镰刀菌和禾谷镰刀菌。
