Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland.
Biomolecules. 2021 Jan 13;11(1):98. doi: 10.3390/biom11010098.
The occurrence and diversity of and in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate and communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 strains, three and five isolates. The assay on solid substrate showed that and can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of and . The production of toxic, insecticidal and antibacterial compounds in cultures of and suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.
玉米种子内生菌群落的发生和多样性及其在该谷物中的作用尚未被充分了解。因此,本研究旨在调查从波兰田间采集的玉米种子内生菌群落,并研究其形成特定生物活性物质的潜力。对内转录间隔区 1(ITS 1)和 2(ITS2)以及核糖体 RNA 基因簇大亚基(LSU,28S)的测序结果鉴定出了 17 株 ,3 株 ,和 5 株 。在固体基质上的检测表明 和 可以合成巴戟醇内酯、Vertilecanin A、Vertilecanin A 甲酯、2-癸烯二酸和 10-羟基-8-癸烯酸。这也是首次揭示这两个物种分别产生 beauvericin 和 enniatin B1 的能力。此外,在本研究中,首次注释 Pyrrocidine A 和/或 B 是 和 的代谢产物。 和 在培养物中产生有毒、杀虫和抗菌化合物,这表明有必要重新研究栖息在玉米种子中的真菌的生物学作用。