Oka H, Ikai Y, Kawamura N, Uno K, Yamada M, Harada K, Suzuki M
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
J Chromatogr. 1987 Jul 29;400:253-61. doi: 10.1016/s0021-9673(01)81619-8.
An analytical system for the simultaneous determination of residual oxytetracycline, tetracycline, chlortetracycline, doxycycline, methacycline, demethylchlortetracycline and minocycline in honey has been established by a combination of simple thin-layer chromatographic (TLC) and precise high-performance liquid chromatographic (HPLC) methods. In this system, screening by TLC can detect tetracyclines (TCs) at a level of 0.1 ppm in honey without the need for special equipment, and the quantitative method by HPLC can determine TCs with good recovery (83.7-99.6%) and coefficient variation (0.9-4.3%).
