柴胡皂苷a、c和d的纯化。大规模反相高效液相色谱法的应用。
Purification of saikosaponins a, c and d. Application of large-scale reversed high-performance liquid chromatography.
作者信息
Sakuma S, Motomura H
机构信息
Chromatographic Division, Kurita Water Industries Ltd., Tokyo, Japan.
出版信息
J Chromatogr. 1987 Jul 29;400:293-5. doi: 10.1016/s0021-9673(01)81624-1.
PMID:3667757
Abstract
The preparative separation of saikosaponins a, c, and d from Bupleurum falcatum was performed by high-performance liquid chromatography on a 100 X 11 cm I.D. axially compressed column, packed with 20-micron ODS silica gel using a step gradient with mixtures of acetonitrile and water as the mobile phase. Gram quantities of pure compounds were obtained in a single run.
摘要
采用高效液相色谱法，在一根内径为100×11厘米的轴向压缩柱上，以20微米的十八烷基硅烷键合硅胶为填充剂，以乙腈和水的混合物为流动相进行梯度洗脱，从柴胡中制备分离柴胡皂苷a、c和d。单次运行即可获得克级量的纯化合物。
Zotero 插件