Salari H
Department of Medicine, University of British Columbia, Vancouver General Hospital Research Institute, Canada.
J Chromatogr. 1987 Aug 7;419:103-11. doi: 10.1016/0378-4347(87)80270-0.
A liquid-liquid extraction technique and six solid absorbents, silica gel, octadecyl silica (C18), XAD-2, XAD-4, XAD-7 and XAD-8, were compared as effective tools for extraction of phosphatidylcholine (Ptd C), phosphatidylethanolamine (Ptd E), phosphatidylinositol (Ptd I) and phosphatidylserine (Ptd S) from plasma. Using liquid-liquid extraction the recovery of the four phospholipids was in the order of 60% in organic phase and 20% in the liquid interface. Neither silica gel nor C18 materials significantly adsorbed phospholipids from plasma. Amberlite resins were more selective for removal of phospholipids. A recovery of greater than 85% was obtained for Ptd C, Ptd I and Ptd S when XAD-7 or XAD-8 were used as adsorbents. A recovery of approximately 90% for Ptd E was obtained when XAD-2 or XAD-4 were used as adsorbents. A solvent mixture of isopropanol-acetonitrile (1:1) was found to be the most effective eluent for removal of phospholipids from amberlite polymeric resins. These results suggest that resins could be used as an extracting tool for removal of phospholipids from body fluids.
比较了液-液萃取技术和六种固体吸附剂（硅胶、十八烷基硅胶（C18）、XAD-2、XAD-4、XAD-7和XAD-8）作为从血浆中萃取磷脂酰胆碱（Ptd C）、磷脂酰乙醇胺（Ptd E）、磷脂酰肌醇（Ptd I）和磷脂酰丝氨酸（Ptd S）的有效工具。采用液-液萃取时，四种磷脂在有机相中的回收率约为60%，在液-液界面的回收率约为20%。硅胶和C18材料均未显著吸附血浆中的磷脂。Amberlite树脂对磷脂的去除更具选择性。当使用XAD-7或XAD-8作为吸附剂时，Ptd C、Ptd I和Ptd S的回收率大于85%。当使用XAD-2或XAD-4作为吸附剂时，Ptd E的回收率约为90%。发现异丙醇-乙腈（1:1）的混合溶剂是从Amberlite聚合树脂中去除磷脂的最有效洗脱剂。这些结果表明，树脂可作为从体液中去除磷脂的萃取工具。
