Phytochemistry Laboratory, National Organization for Drug Control and Research, Giza 12622, Egypt.
Phytochemistry and Plant Systematics Department, National Research Centre, Dokki, Cairo 12622, Egypt.
Molecules. 2023 Jan 9;28(2):674. doi: 10.3390/molecules28020674.
is a genus compromising over 250 herbaceous flowering species and is used traditionally to treat several ailments. Among the Egyptian species, was reported to be cytotoxic against multidrug-resistant cancer cells. In this context, we aimed to explore the metabolome of and compare it to other members of the genus in pursuance of identifying its bioactive principles. An LC-MS/MS analysis approach synchronized with feature-based molecular networks was adopted to offer a holistic overview of the metabolome diversity of the Egyptian species. The studied plants included and . Their constitutive metabolome showed diverse chemical classes such as cinnamic acids, sesquiterpene lactones, flavonoids, and lignans. Linking the recorded metabolome to the previously reported cytotoxicity identified sesquiterpene lactones as the major contributors to this activity. To confirm our findings, bioassay-guided fractionation of was adopted and led to the isolation of the sesquiterpene lactone cynaropicrin with an IC of 1.817 µM against the CCRF-CEM leukemia cell line. The adopted methodology highlighted the uniqueness of the constitutive metabolome of and determined the sesquiterpene lactones to be the responsible cytotoxic metabolites.
是一个包含超过 250 种草本开花物种的属，传统上用于治疗多种疾病。在埃及物种中， 被报道对多药耐药癌细胞具有细胞毒性。在这方面，我们旨在探索 的代谢组学，并将其与属中的其他成员进行比较，以确定其生物活性成分。采用 LC-MS/MS 分析方法与基于特征的分子网络同步，对埃及 物种的代谢组学多样性进行了全面概述。所研究的植物包括 和 。它们的组成代谢组显示出多种化学类群，如肉桂酸、倍半萜内酯、类黄酮和木脂素。将记录的代谢组与先前报道的细胞毒性联系起来，确定倍半萜内酯是这种活性的主要贡献者。为了证实我们的发现，采用了基于生物测定的 分离，导致分离出倍半萜内酯 Cynaropicrin，对 CCRF-CEM 白血病细胞系的 IC 为 1.817 µM。所采用的方法强调了 的组成代谢组的独特性，并确定倍半萜内酯是负责细胞毒性的代谢物。
