Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, 12622 Giza, Egypt.
Biomolecules. 2020 Jul 4;10(7):1000. doi: 10.3390/biom10071000.
The popular beverage green tea possesses chemopreventive activity against various types of tumors. However, the effects of its chemopreventive effect on hematological malignancies have not been defined. In the present study, we evaluated antitumor efficacies of a specific green tea, sencha tea, on sensitive and multidrug-resistant leukemia and a panel of nine multiple myelomas (MM) cell lines. We found that sencha extracts induced cytotoxicity in leukemic cells and MM cells to different extents, yet its effect on normal cells was limited. Furthermore, sencha extracts caused G2/M and G0/G1 phase arrest during cell cycle progression in CCRF/CEM and KMS-12-BM cells, respectively. Specifically, sencha-MeOH/HO extracts induced apoptosis, ROS, and MMP collapse on both CCRF/CEM and KMS-12-BM cells. The analysis with microarray and COMPARE in 53 cell lines of the NCI panel revealed diverse functional groups, including cell morphology, cellular growth and proliferation, cell cycle, cell death, and survival, which were closely associated with anti-tumor effects of sencha tea. It is important to note that PI3K/Akt and NF-κB pathways were the top two dominant networks by ingenuity pathway analysis. We demonstrate here the multifactorial modes of action of sencha tea leading to chemopreventive effects of sencha tea against cancer.
绿茶是一种广受欢迎的饮品，具有预防多种肿瘤的作用。然而，其对血液系统恶性肿瘤的化学预防作用尚未明确。在本研究中，我们评估了一种特定绿茶——煎茶对敏感和多药耐药白血病以及一组 9 种多发性骨髓瘤（MM）细胞系的抗肿瘤功效。我们发现，煎茶提取物在不同程度上诱导白血病细胞和 MM 细胞发生细胞毒性，但对正常细胞的作用有限。此外，煎茶提取物分别导致 CCRF/CEM 和 KMS-12-BM 细胞的细胞周期进程中 G2/M 和 G0/G1 期阻滞。具体而言，煎茶-MeOH/HO 提取物诱导 CCRF/CEM 和 KMS-12-BM 细胞凋亡、ROS 和 MMP 崩溃。对 NCI 面板 53 个细胞系的微阵列和 COMPARE 分析显示，包括细胞形态、细胞生长和增殖、细胞周期、细胞死亡和存活在内的多个功能群与煎茶的抗肿瘤作用密切相关。需要注意的是，PI3K/Akt 和 NF-κB 通路是 Ingenuity 通路分析中排名前两位的主导网络。我们在此证明了煎茶具有多种作用模式，可导致其发挥防癌作用。
