Pollastri Susanna, Velikova Violeta, Castaldini Maurizio, Fineschi Silvia, Ghirardo Andrea, Renaut Jenny, Schnitzler Jörg-Peter, Sergeant Kjell, Winkler Jana Barbro, Zorzan Simone, Loreto Francesco
Institute for Sustainable Plant Protection (IPSP), National Research Council of Italy (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence, Italy.
Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
Plants (Basel). 2023 Jan 11;12(2):333. doi: 10.3390/plants12020333.
Isoprene-emitting plants are better protected against thermal and oxidative stresses, which is a desirable trait in a climate-changing (drier and warmer) world. Here we compared the ecophysiological performances of transgenic isoprene-emitting and wild-type non-emitting tobacco plants during water stress and after re-watering in actual environmental conditions (400 ppm of CO and 28 °C of average daily temperature) and in a future climate scenario (600 ppm of CO and 32 °C of average daily temperature). Furthermore, we intended to complement the present knowledge on the mechanisms involved in isoprene-induced resistance to water deficit stress by examining the proteome of transgenic isoprene-emitting and wild-type non-emitting tobacco plants during water stress and after re-watering in actual climate. Isoprene emitters maintained higher photosynthesis and electron transport rates under moderate stress in future climate conditions. However, physiological resistance to water stress in the isoprene-emitting plants was not as marked as expected in actual climate conditions, perhaps because the stress developed rapidly. In actual climate, isoprene emission capacity affected the tobacco proteomic profile, in particular by upregulating proteins associated with stress protection. Our results strengthen the hypothesis that isoprene biosynthesis is related to metabolic changes at the gene and protein levels involved in the activation of general stress defensive mechanisms of plants.
释放异戊二烯的植物能更好地抵御热胁迫和氧化胁迫，在气候变化（更干燥、更温暖）的世界中，这是一个理想的特性。在此，我们比较了转基因释放异戊二烯烟草植株和野生型不释放异戊二烯烟草植株在实际环境条件（400 ppm二氧化碳和28℃日平均温度）以及未来气候情景（600 ppm二氧化碳和32℃日平均温度）下水分胁迫期间及复水后的生理生态表现。此外，我们打算通过研究转基因释放异戊二烯烟草植株和野生型不释放异戊二烯烟草植株在实际气候条件下水分胁迫期间及复水后的蛋白质组，来补充目前关于异戊二烯诱导的对水分亏缺胁迫抗性所涉及机制的知识。在未来气候条件下的中度胁迫下，释放异戊二烯的植株维持了较高的光合作用和电子传递速率。然而，在实际气候条件下，释放异戊二烯的植株对水分胁迫的生理抗性并不像预期的那么明显，这可能是因为胁迫发展迅速。在实际气候中，异戊二烯排放能力影响了烟草的蛋白质组图谱，特别是通过上调与胁迫保护相关的蛋白质。我们的结果强化了这样一种假设，即异戊二烯生物合成与植物一般胁迫防御机制激活过程中涉及的基因和蛋白质水平的代谢变化有关。
