Zhang Ling, Shi Xueli, Hou Huaxi, Lin Qinyuan, Zhu Shijiang, Wang Guang
Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Plants (Basel). 2023 Jan 11;12(2):334. doi: 10.3390/plants12020334.
The cytokinin 6-benzyladenine (6-BA) is widely used to regulate the growth of horticultural crops. However, it is not clear how postharvest treatment with 6-BA at various concentrations affects the quality of Chinese flowering cabbage. In this study, harvested Chinese flowering cabbage was foliar sprayed with 6-BA solution at concentrations of 5, 10, 20, 40, and 80 mg·L. All 6-BA treatments protected the quality of Chinese flowering cabbage during storage, and the treatment with 20 and 40 mg·L 6-BA showed the most obvious effect. Treatment with 6-BA reduced leaf yellowing degree and weight loss rate; maintained high chlorophyll and chlorophyll contents; suppressed the declines in ascorbic acid and soluble protein; enhanced antioxidant capacity; and reduced oxidative damage in cabbage leaves. Furthermore, 6-BA treatment upregulated the expression of antioxidant genes and the activities of SOD, POD, and CAT, while inhibiting the expression of senescence-related gene () and chlorophyll catabolic genes (, , , , ). These results suggest that postharvest 6-BA treatment enhances antioxidant capacity, delays leaf senescence, and inhibits chlorophyll degradation, thereby maintaining the quality of Chinese flowering cabbage during storage. The findings of this study provide a candidate method for preserving Chinese flowering cabbage after harvest.
细胞分裂素6-苄基腺嘌呤(6-BA)被广泛用于调控园艺作物的生长。然而,目前尚不清楚不同浓度的6-BA采后处理对菜心品质有何影响。在本研究中,对采收后的菜心进行叶面喷施浓度为5、10、20、40和80 mg·L的6-BA溶液处理。所有6-BA处理均在贮藏期间保持了菜心的品质,其中20和40 mg·L 6-BA处理效果最为明显。6-BA处理降低了叶片黄化程度和失重率;维持了较高的叶绿素a和叶绿素b含量;抑制了抗坏血酸和可溶性蛋白的下降;增强了抗氧化能力;减少了叶片的氧化损伤。此外,6-BA处理上调了抗氧化基因的表达以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,同时抑制了衰老相关基因()和叶绿素分解代谢基因(、、、、)的表达。这些结果表明,采后6-BA处理增强了抗氧化能力,延缓了叶片衰老,抑制了叶绿素降解,从而在贮藏期间保持了菜心的品质。本研究结果为采后菜心保鲜提供了一种候选方法。