Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Food Res Int. 2023 Jan;163:112229. doi: 10.1016/j.foodres.2022.112229. Epub 2022 Nov 24.
Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) is a candidate of selenium (Se) accumulator, but it is not clear whether and how preharvest Se treatment affects its quality after harvest. Here, we showed that preharvest application of 100 μmol/L selenite to roots enhanced storage quality of Chinese flowering cabbage. It increased antioxidant capacity and reduced weight loss, leaf yellowing, and protein degradation after harvest. Furthermore, it increased the activities of antioxidant enzymes such as POD, CAT, GSH-Px, and GR, as well as contents of AsA, GSH, phenolics, and flavonoids during storage. Metabolome analysis revealed that phenolic acids including p-Coumaric acid, caffeic acid, and ferulic acid; flavonoids such as naringenin, eriodictyol, apigenin, quercetin, kaempferol, and their derivatives were notably increased by preharvest selenite treatment. Consistently, the total antioxidant capacity, evaluated by DPPH, ABTS, and FRAP methods, were all markedly enhanced in selenite-treated cabbage compared to the control. Transcriptomics analysis showed that the DEGs induced by selenite were significantly enriched in AsA-GSH metabolisms and phenylpropanoids biosynthesis pathways. Moreover, preharvest selenite treatment significantly up-regulated the expressions of BrGST, BrGSH-Px, BrAPX, BrASO, BrC4H, BrCOMT, BrCHS, and BrFLS during storage. These results suggest that preharvest selenite treatment enhanced quality of cabbage not only by increasing Se biological accumulation, but also through regulating AsA-GSH cycle and increasing phenolics and flavonoids synthesis after harvest. This study provides a novel insight into the effects of preharvest Se treatment on quality of Chinese flowering cabbage during storage.
白菜花（ Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee）是硒（Se）的积累候选者，但尚不清楚收获前施用硒是否以及如何影响其收获后的品质。在这里，我们表明，将 100μmol/L 亚硒酸盐施用于根部可增强白菜花的贮藏品质。它增加了抗氧化能力，并减少了收获后叶片的失重、黄化和蛋白质降解。此外，它还增加了 POD、CAT、GSH-Px 和 GR 等抗氧化酶的活性，以及 AsA、GSH、酚类和类黄酮在贮藏期间的含量。代谢组学分析表明，通过收获前亚硒酸盐处理，包括对香豆酸、咖啡酸和阿魏酸在内的酚酸；柚皮素、圣草酚、芹菜素、槲皮素、山奈酚和它们的衍生物在内的类黄酮显著增加。一致地，通过 DPPH、ABTS 和 FRAP 方法评估的总抗氧化能力在亚硒酸盐处理的白菜中均明显高于对照。转录组学分析表明，亚硒酸盐诱导的 DEGs 在 AsA-GSH 代谢和苯丙烷生物合成途径中显著富集。此外，收获前亚硒酸盐处理在贮藏过程中显著上调了 BrGST、BrGSH-Px、BrAPX、BrASO、BrC4H、BrCOMT、BrCHS 和 BrFLS 的表达。这些结果表明，收获前的亚硒酸盐处理不仅通过增加 Se 的生物积累，而且通过调节 AsA-GSH 循环和增加收获后酚类和类黄酮的合成来增强白菜的品质。本研究为收获前 Se 处理对白菜花贮藏品质的影响提供了新的见解。
