Liu Tiantian, Li Yuxin, Wang Chang, Zhang Da, Liu Jiajia, He Mingyuan, Chen Mingxun, Guo Yuan
National Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis, and College of Agronomy, Northwest A&F University, Yangling 712100, China.
Plants (Basel). 2023 Jan 11;12(2):347. doi: 10.3390/plants12020347.
Leaf senescence is the final stage of leaf development and is essential for storage properties and crop productivity. WRKY transcription factors have been revealed to play crucial roles in several biological processes during plant growth and development, especially in leaf senescence. However, the functions of WRKY transcription factors in leaf senescence remain unclear. In the present study, , one paralogue of , was cloned from the cultivar "Zhongshuang11 (ZS11)". We found that Bna.A07.WRKY70 contains a highly conserved WRKY domain and is most closely related to WRKY70. The subcellular localization and transcriptional self-activation assays indicated that Bna.A07.WRKY70 functions as a transcription factor. Meanwhile, RT-qPCR and promoter-GUS analysis showed that is predominantly expressed in the leaves of and rosette leaves of . In addition, our results demonstrated that ectopic expression of in mutants could restore the senescence phenotypes to wild-type levels. Consistently, the expression levels of three senescence-related marker genes of mutants were restored to wild-type levels by ectopic expression of . These findings improve our understanding of the function of in and provide a novel strategy for breeding the new stay-green cultivars in rapeseed through genetic manipulation.
叶片衰老为叶片发育的最后阶段，对贮藏特性和作物生产力至关重要。WRKY转录因子在植物生长发育的多个生物学过程中发挥关键作用，尤其是在叶片衰老过程中。然而，WRKY转录因子在叶片衰老中的功能仍不清楚。在本研究中，从甘蓝型油菜品种“中双11号（ZS11）”中克隆了一个的旁系同源基因。我们发现Bna.A07.WRKY70含有一个高度保守的WRKY结构域，并且与WRKY70关系最为密切。亚细胞定位和转录自激活分析表明Bna.A07.WRKY70作为转录因子发挥作用。同时，RT-qPCR和启动子-GUS分析表明在的叶片和莲座叶中大量表达。此外，我们的结果表明，在突变体中异位表达可以将衰老表型恢复到野生型水平。一致地，通过异位表达，突变体的三个衰老相关标记基因的表达水平恢复到野生型水平。这些发现增进了我们对在中的功能的理解，并为通过基因操作培育油菜新的持绿品种提供了新策略。
