Camargo Paula Oliveira, Calzado Natália Fermino, Budzinski Ilara Gabriela Frasson, Domingues Douglas Silva
Group of Genomics and Transcriptomes in Plants, Department of Biodiversity, Institute of Biosciences, São Paulo State University, UNESP, Rio Claro 13506-900, SP, Brazil.
Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", University of São Paulo, USP, Piracicaba 13418-900, SP, Brazil.
Plants (Basel). 2023 Jan 14;12(2):398. doi: 10.3390/plants12020398.
Lipoxygenases (LOXs) are enzymes that catalyze the addition of an oxygen molecule to unsaturated fatty acids, thus forming hydroperoxides. In plants, these enzymes are encoded by a multigene family found in several organs with varying activity patterns, by which they are classified as LOX9 or LOX13. They are involved in several physiological functions, such as growth, fruit development, and plant defense. Despite several studies on genes of the LOX family in plants, most studies are restricted to a single species or a few closely related species. This study aimed to analyze the diversity, evolution, and expression of LOX genes in angiosperm species. We identified 247 LOX genes among 23 species of angiosperms and basal plants. Phylogenetic analyses identified clades supporting LOX13 and two main clades for LOX9: LOX9_A and LOX9_B. Eudicot species such as , , and did not present LOX9_B genes; however, LOX9_B was present in all monocots used in this study. We identified that there were potential new subcellular localization patterns and conserved residues of oxidation for LOX9 and LOX13 yet unexplored. In summary, our study provides a basis for the further functional and evolutionary study of lipoxygenases in angiosperms.
脂氧合酶（LOXs）是一类催化将氧分子添加到不饱和脂肪酸上，从而形成氢过氧化物的酶。在植物中，这些酶由一个多基因家族编码，该家族存在于几个具有不同活性模式的器官中，据此它们被分类为LOX9或LOX13。它们参与多种生理功能，如生长、果实发育和植物防御。尽管对植物中LOX家族的基因进行了多项研究，但大多数研究仅限于单个物种或少数几个密切相关的物种。本研究旨在分析被子植物物种中LOX基因的多样性、进化和表达。我们在23种被子植物和基部植物中鉴定出了247个LOX基因。系统发育分析确定了支持LOX13的分支以及LOX9的两个主要分支：LOX9_A和LOX9_B。诸如 、 和 等双子叶植物物种没有LOX9_B基因；然而，本研究中使用的所有单子叶植物中都存在LOX9_B。我们发现LOX9和LOX13存在尚未探索的潜在新亚细胞定位模式和氧化保守残基。总之，我们的研究为被子植物中脂氧合酶的进一步功能和进化研究提供了基础。
