Wang Licong, Yang Maoxia, Luo Sheng, Yang Guanjun, Lu Xinjiang, Lu Jianfei, Chen Jiong
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
Vaccines (Basel). 2023 Jan 16;11(1):186. doi: 10.3390/vaccines11010186.
Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of herpesviral hematopoietic necrosis (HVHN) disease, which causes serious economic losses in the crucian carp culture industry. In this study, by displaying ORF132 on the surface of cells (named EBY100/pYD1-ORF132), we evaluated the protective efficacy of oral administration against CyHV-2 infection. Intense innate and adaptive immune responses were evoked in both mucosal and systemic tissues after oral vaccination with EBY100/pYD1-ORF132. Importantly, oral vaccination provided significant protection for crucian carp post CyHV-2 infection, resulting in a relative percent survival (RPS) of 64%. In addition, oral administration suppressed the virus load and relieved histological damage in selected tissues. Our results indicated that surface-displayed ORF132 on could be used as potential oral vaccine against CyHV-2 infection.
鲤疱疹病毒2（CyHV-2）是疱疹病毒性造血坏死（HVHN）疾病的病原体，该疾病给鲫鱼养殖业造成了严重的经济损失。在本研究中，通过在细胞表面展示开放阅读框132（命名为EBY100/pYD1-ORF132），我们评估了口服给药对CyHV-2感染的保护效果。用EBY100/pYD1-ORF132口服疫苗接种后，在黏膜和全身组织中均引发了强烈的先天性和适应性免疫反应。重要的是，口服疫苗为CyHV-2感染后的鲫鱼提供了显著保护，相对存活率（RPS）达到64%。此外，口服给药抑制了病毒载量，并减轻了所选组织的组织学损伤。我们的结果表明，细胞表面展示的ORF132可作为预防CyHV-2感染的潜在口服疫苗。
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