Ghoneum M, Grimes P E, Gill G, Kelly A P
Department of Otolaryngology, Charles R. Drew Postgraduate Medical School, Los Angeles, CA.
J Am Acad Dermatol. 1987 Oct;17(4):600-5. doi: 10.1016/s0190-9622(87)70243-6.
Natural cell-mediated cytotoxicity was studied in 18 patients with vitiligo and 13 healthy age-, race-, and sex-matched control subjects. The 4-hour chromium51 (51Cr) release assay was used to determine the activity of natural killer (NK) cells in the peripheral blood against K562 and Molt-4 target cells. Patients with vitiligo had a 50%, 67%, and 60% decrease in the cytotoxic response with Molt-4 cells at effector-target ratios of 25:1, 50:1, and 100:1, respectively, in comparison with control subjects (p less than 0.001). This inhibition was consistent with an 80% decrease in the binding capacity of NK cells to Molt-4 target cells (p less than 0.005). In contrast, cytotoxic responses did not differ in patients and control subjects with K562 target cells. These results suggest that patients with vitiligo have a decreased capacity for effector cell recognition of Molt-4 target cells but not K562 target cells. Hence patients with vitiligo may have defective clones of NK cells that are incapable of initial recognition of Molt-4 target cells, a necessary prerequisite for target cell lysis. Perhaps this phenomenon occurs with other tumor cells, which would explain the association of vitiligo with certain internal malignancies.
对18例白癜风患者和13名年龄、种族及性别相匹配的健康对照者进行了自然细胞介导的细胞毒性研究。采用4小时铬51（51Cr）释放试验来测定外周血中自然杀伤（NK）细胞对K562和Molt-4靶细胞的活性。与对照者相比，白癜风患者在效应细胞与靶细胞比例为25:1、50:1和100:1时，对Molt-4细胞的细胞毒性反应分别降低了50%、67%和60%（p<0.001）。这种抑制作用与NK细胞对Molt-4靶细胞的结合能力降低80%相一致（p<0.005）。相比之下，白癜风患者和对照者对K562靶细胞的细胞毒性反应没有差异。这些结果表明，白癜风患者对Molt-4靶细胞的效应细胞识别能力降低，但对K562靶细胞的识别能力未降低。因此，白癜风患者可能存在NK细胞克隆缺陷，无法初始识别Molt-4靶细胞，而这是靶细胞裂解的必要前提。也许这种现象也发生在其他肿瘤细胞上，这可以解释白癜风与某些内部恶性肿瘤的关联。
