白癜风中自然杀伤细胞和淋巴因子激活的杀伤细胞对黑素细胞的活性
Natural killer cell and lymphokine-activated killer cell activity against melanocytes in vitiligo.
作者信息
Durham-Pierre D G, Walters C S, Halder R M, Pham H N, Vanderpool E A
机构信息
Department of Microbiology, Howard University College of Medicine, Washington, DC 20060, USA.
出版信息
J Am Acad Dermatol. 1995 Jul;33(1):26-30. doi: 10.1016/0190-9622(95)90005-5.
BACKGROUND
Vitiligo is a common disease of unknown cause. Previous studies have shown abnormalities in natural killer (NK) cell cytotoxicity in patients when NK-sensitive erythroleukemic cell lines were used as target cells.
OBJECTIVE
The purpose of this study was to use melanocytes directly as target cells to determine NK and lymphokine-activated killer (LAK) cell cytotoxicity in patients with vitiligo and to determine whether NK or LAK cells can be implicated in any destructive mechanism for melanocyte cytotoxicity in vitro in this disease.
METHODS
Twenty-one patients with vitiligo were compared with a control group by studying NK cell activity (NKCA) and LAK cell activity (LAKCA) on several target cells. These included K562 cells, neonatal melanocytes, and malignant melanoma cells for NKCA and neonatal melanocytes and malignant melanoma cells for LAKCA. Cytotoxicity was measured with the standard chromium 51-release assay.
RESULTS
No significant differences were found between vitiligo patients and control subjects in NKCA against K562 cells or in NKCA and LAKCA against melanocytes.
CONCLUSION
NK cells and LAK cells are probably not responsible for melanocyte destruction in vitiligo.
背景
白癜风是一种病因不明的常见疾病。以往研究表明，当使用对自然杀伤（NK）细胞敏感的红白血病细胞系作为靶细胞时，白癜风患者的NK细胞细胞毒性存在异常。
目的
本研究旨在直接使用黑素细胞作为靶细胞，测定白癜风患者的NK细胞和淋巴因子激活的杀伤（LAK）细胞的细胞毒性，并确定在该疾病中，NK细胞或LAK细胞是否参与体外黑素细胞细胞毒性的任何破坏机制。
方法
通过研究21例白癜风患者对几种靶细胞的NK细胞活性（NKCA）和LAK细胞活性（LAKCA），并与对照组进行比较。这些靶细胞包括用于NKCA检测的K562细胞、新生儿黑素细胞和恶性黑色素瘤细胞，以及用于LAKCA检测的新生儿黑素细胞和恶性黑色素瘤细胞。细胞毒性采用标准的铬51释放试验进行测定。
结果
白癜风患者与对照组在针对K562细胞的NKCA方面，以及在针对黑素细胞的NKCA和LAKCA方面，均未发现显著差异。
结论
NK细胞和LAK细胞可能与白癜风中黑素细胞的破坏无关。
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