首次在夏威夷自然感染的 spp. 中检测到一种新的 RNA 病毒——芙蓉贝塔呼肠孤病毒和一种新的 DNA 病毒——芙蓉大豆花叶病毒，并对其进行了基因组特征分析。

First Detection and Genome Characterization of a New RNA Virus, Hibiscus Betacarmovirus, and a New DNA Virus, Hibiscus Soymovirus, Naturally Infecting spp. in Hawaii.

机构信息

Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822, USA.

United States Department of Agriculture Agricultural Research Service, U.S. Pacific Basin Agricultural Research Center, Hilo, HI 96720, USA.

出版信息

Viruses. 2022 Dec 29;15(1):90. doi: 10.3390/v15010090.

DOI:10.3390/v15010090

PMID:36680129

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9865081/

Abstract

Hibiscus ( spp., family Malvaceae) leaves exhibiting symptoms of mosaic, ringspot, and chlorotic spots were collected in 2020 on Oahu, HI. High-throughput sequencing analysis was conducted on ribosomal RNA-depleted composite RNA samples extracted from symptomatic leaves. About 77 million paired-end reads and 161,970 contigs were generated after quality control, trimming, and de novo assembly. Contig annotation with BLASTX/BLASTN searches revealed a sequence (contig 1) resembling the RNA virus, hibiscus chlorotic ringspot virus (genus ), and one (contig 2) resembling the DNA virus, peanut chlorotic streak virus (genus ). Further bioinformatic analyses of the complete viral genome sequences indicated that these viruses, with proposed names of hibiscus betacarmovirus and hibiscus soymovirus, putatively represent new species in the genera and , respectively. RT-PCR using specific primers, designed based on the retrieved contigs, coupled with Sanger sequencing, further confirmed the presence of these viruses. An additional 54 hibiscus leaf samples from other locations on Oahu were examined to determine the incidence and distribution of these viruses.

摘要

2020 年，在夏威夷瓦胡岛采集到表现花叶、环斑和黄化斑点症状的芙蓉属（ spp.，锦葵科）叶片。从有症状的叶片中提取核糖体 RNA 耗尽的复合 RNA 样品进行高通量测序分析。经过质量控制、修剪和从头组装后，产生了约 7700 万对末端读取和 161970 个连续序列。使用 BLASTX/BLASTN 搜索对连续序列进行注释，揭示了一个与 RNA 病毒（芙蓉黄脉环斑病毒，属）相似的序列（连续序列 1）和一个与 DNA 病毒（花生黄脉斑点病毒，属）相似的序列（连续序列 2）。对完整病毒基因组序列的进一步生物信息学分析表明，这些病毒，暂定名为芙蓉 betacarmovirus 和芙蓉大豆花叶病毒，分别代表属和属中的新种。基于检索到的连续序列设计的特异性引物的 RT-PCR 与 Sanger 测序相结合，进一步证实了这些病毒的存在。对瓦胡岛其他地点的另外 54 个芙蓉叶片样本进行了检测，以确定这些病毒的发生率和分布情况。