Momeni Sara, Shanei Ahmad, Sazgarnia Ameneh, Attaran Neda, Aledavood Seyed Amir
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Email:
Cell J. 2023 Jan 1;25(1):51-61. doi: 10.22074/cellj.2022.559078.1097.
The multimodality treatment of cancer provides a secure and effective approach to improve the outcome of treatments. Cold atmospheric plasma (CAP) has got attention because of selectively target and kills cancer cells. Likewise, gold nanoparticles (GNP) have been introduced as a radiosensitizer and drug delivery with high efficacy and low toxicity in cancer treatment. Conjugating GNP with indocyanine green (ICG) can develop a multifunctional drug to enhance radio and photosensitivity. The purpose of this study is to evaluate the anticancer effects of GNP@ICG in radiotherapy (RT) and CAP on DFW melanoma cancer and HFF fibroblast normal cell lines.
In this experimental study, the cells were irradiated to RT and CAP, alone and in combination with or without GNP@ICG at various time sequences between RT and CAP. Apoptosis Annexin V/PI, MTT, and colony formation assays evaluated the therapeutic effect. Finally, the index of synergism was calculated to compare the results.
Most crucially, the cell viability assay showed that RT was less toxic to tumors and normal cells, but CAP showed a significant anti-tumor effect on melanoma cells with selective toxicity. In addition, cold plasma sensitized melanoma cells to radiotherapy so increasing treatment efficiency. This effect is enhanced with GNP@ICG. In comparison to RT alone, the data showed that combination treatment greatly decreased monolayer cell colonization and boosted apoptotic induction.
The results provide new insights into the development of better approaches in radiotherapy of melanoma cells assisted plasma and nanomedicine.
癌症的多模态治疗提供了一种安全有效的方法来改善治疗结果。冷大气等离子体（CAP）因其能选择性地靶向并杀死癌细胞而受到关注。同样，金纳米颗粒（GNP）已被引入作为一种放射增敏剂和药物递送载体，在癌症治疗中具有高效低毒的特点。将GNP与吲哚菁绿（ICG）结合可以开发出一种多功能药物，以增强放射敏感性和光敏感性。本研究的目的是评估GNP@ICG在放疗（RT）和CAP对DFW黑色素瘤癌细胞和HFF成纤维细胞正常细胞系中的抗癌作用。
在本实验研究中，将细胞分别单独以及在RT和CAP之间的不同时间顺序下联合或不联合GNP@ICG进行RT和CAP照射。通过凋亡膜联蛋白V/PI、MTT和集落形成试验评估治疗效果。最后，计算协同指数以比较结果。
最关键的是，细胞活力测定表明RT对肿瘤细胞和正常细胞的毒性较小，但CAP对黑色素瘤细胞具有显著的抗肿瘤作用且具有选择性毒性。此外，冷等离子体使黑色素瘤细胞对放疗敏感，从而提高了治疗效率。GNP@ICG可增强这种效果。与单独的RT相比，数据表明联合治疗大大降低了单层细胞集落形成并增强了凋亡诱导。
这些结果为在等离子体辅助和纳米医学辅助下开发更好的黑色素瘤细胞放疗方法提供了新的见解。
