有机锌适中的螯合强度可增强肉鸡空肠和回肠中相关转运蛋白的表达。
The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers.
机构信息
Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou 225000, People's Republic of China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
出版信息
Poult Sci. 2023 Mar;102(3):102477. doi: 10.1016/j.psj.2023.102477. Epub 2023 Jan 6.
Our previous study demonstrated that the zinc (Zn) proteinate with moderate chelation strength (Zn-Prot M) enhanced the Zn absorption in the small intestine partially via increasing the expression of some Zn and amino acid transporters in the duodenum of broilers. However, it remains unknown whether the Zn-Prot M could also regulate the expression of related transporters in the jejunum and ileum of broilers in the above enhancement of Zn absorption. The present study was conducted to investigate the effect of the Zn-Prot M on the expression of related transporters in the jejunum and ileum of broilers compared to the Zn sulfate (ZnS). Zinc-deficient broilers (13-d-old) were fed with the Zn-unsupplemented basal diets (control) or the basal diets supplemented with 60 mg Zn/kg as ZnS or Zn-Prot M for 26 d. The results showed that in the jejunum, compared to the control, supplementation of the organic or inorganic Zn increased (P < 0.05) mRNA and protein expression of b-type amino acid transporter (rBAT), Zn transporter 10 (ZnT10), and peptide-transporter 1 (PepT1) mRNA expression and Zn transporter 7 (ZnT7) protein expression on d 28, while y+L-type amino transporter 2 (y+LAT2) mRNA and protein expression, and protein expression of ZnT7 and ZnT10 on 28 d and zrt-irt-like protein 3 (ZIP3) and zrt-irt-like protein 5 (ZIP5) on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. In the ileum, Zn addition regardless of Zn source up-regulated (P < 0.05) mRNA expression of Zn transporter 9 (ZnT9) and ZIP3, ZIP5, and y+LAT2 protein expression on d 28, and PepT1 mRNA and protein expression, ZIP3 and y+LAT2 mRNA expression and ZnT10 protein expression on d 39. Furthermore, Zn transporter 4 (ZnT4) and ZnT9 mRNA expression and Zn transporter 1 (ZnT1) protein expression on d 28, and y+LAT2 mRNA expression and ZnT10 and PepT1 protein expression on d 39 were higher (P < 0.05) for Zn-Prot M than for ZnS. It was concluded that the Zn-Prot M enhanced the expression of the ZnT1, ZnT4, ZnT9, ZnT10, ZIP3, ZIP5, y+LAT2, and PepT1 in the jejunum or ileum of broilers compared to the ZnS.
我们之前的研究表明，中等螯合强度的锌（Zn）蛋白盐（Zn-Prot M）通过增加十二指肠中一些 Zn 和氨基酸转运蛋白的表达，部分增强了肉鸡的小肠 Zn 吸收。然而，目前尚不清楚 Zn-Prot M 是否也可以调节肉鸡空肠和回肠中相关转运蛋白的表达，以增强上述 Zn 吸收。本研究旨在研究 Zn-Prot M 对肉鸡空肠和回肠中相关转运蛋白表达的影响，并与硫酸锌（ZnS）进行比较。13 日龄缺锌肉鸡（缺锌）用不含 Zn 的基础日粮（对照组）或基础日粮添加 60mg/kg Zn 作为 ZnS 或 Zn-Prot M 喂养 26 天。结果表明，与对照组相比，有机或无机 Zn 的补充在第 28 天增加了 b 型氨基酸转运体（rBAT）、Zn 转运蛋白 10（ZnT10）和肽转运蛋白 1（PepT1）mRNA 表达和 Zn 转运蛋白 7（ZnT7）蛋白表达，而在第 28 天 y+L 型氨基酸转运体 2（y+LAT2）mRNA 和蛋白表达，ZnT7 和 ZnT10 蛋白表达，以及第 39 天 zrt-irt-like protein 3（ZIP3）和 zrt-irt-like protein 5（ZIP5）mRNA 表达较高（P<0.05）；Zn-Prot M 比 ZnS 高。在回肠中，无论 Zn 来源如何，Zn 的添加均在第 28 天上调了 Zn 转运蛋白 9（ZnT9）和 ZIP3、ZIP5 和 y+LAT2 蛋白表达，以及 PepT1mRNA 和蛋白表达、ZIP3 和 y+LAT2mRNA 表达和 ZnT10 蛋白表达，在第 39 天还上调了 Zn 转运蛋白 4（ZnT4）和 ZnT9mRNA 表达以及 Zn 转运蛋白 1（ZnT1）蛋白表达，y+LAT2mRNA 表达以及 ZnT10 和 PepT1 蛋白表达（P<0.05）。此外，Zn-Prot M 比 ZnS 还提高了第 28 天 ZnT1、ZnT4、ZnT9、ZnT10、ZIP3、ZIP5、y+LAT2 和 PepT1mRNA 表达和第 39 天 y+LAT2mRNA 表达和 ZnT10 和 PepT1 蛋白表达（P<0.05）。综上所述，Zn-Prot M 增强了肉鸡空肠或回肠中 ZnT1、ZnT4、ZnT9、ZnT10、ZIP3、ZIP5、y+LAT2 和 PepT1 的表达，与 ZnS 相比。
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