Department of Animal and Poultry Sciences, Virginia Tech University, Blacksburg 24061.
Poult Sci. 2018 Jan 1;97(1):313-318. doi: 10.3382/ps/pex262.
Male chickens grow faster than female chickens, which may be due to greater nutrient uptake. The transport of nutrients from the intestine to the blood is mediated by transporters located on the surface of epithelial cells lining the villi. The objective of this study was to profile the mRNA expression of an aminopeptidase and selected amino acid and monosaccharide transporters in the duodenum, jejunum, and ileum of male and female chickens at d of hatch (doh) and at d 7 and d 14 post hatch. The mRNA abundance of aminopeptidase N (APN), a peptide (PepT1), 6 amino acid transporters (ASCT1, bo,+AT, CAT1, EAAT3, LAT1, and y+LAT2), and 3 monosaccharide (GLUT2, GLUT5, and SGLT1) transporters was assayed by real-time PCR. Data were analyzed by ANOVA using JMP Pro11. The abundance of bo,+AT, EAAT3, ASCT1, y+LAT2, and GLUT2 mRNA was greater in male than female chickens (P < 0.05). There was no difference in expression between males and females for the other 5 transporters and APN. There was a sex x age interaction for bo,+AT, PepT1, SGLT1, ASCT1, and y+LAT2 mRNA, with greater mRNA abundance in males than females at doh but no difference between males and females at d 7 and d 14. A 3-way sex x age x tissue interaction was observed for GLUT2 mRNA. There was greater GLUT2 mRNA abundance for males in the duodenum and ileum at doh and in the jejunum at d 7, but no difference between males and females at d 14. Thus, there was differential expression of some nutrient transporters in male and female chicks at doh but not at later ages.
公鸡比母鸡生长速度快，这可能是由于它们吸收更多的营养。营养物质从肠道向血液的转运是由位于绒毛上皮细胞表面的转运体介导的。本研究的目的是在孵化后第 d (doh)和第 7 天和第 14 天，分析雄性和雌性小鸡十二指肠、空肠和回肠中一种氨肽酶和选定的氨基酸及单糖转运体的 mRNA 表达谱。采用实时 PCR 法检测氨肽酶 N (APN)、肽 (PepT1)、6 种氨基酸转运体 (ASCT1、bo,+AT、CAT1、EAAT3、LAT1 和 y+LAT2) 和 3 种单糖 (GLUT2、GLUT5 和 SGLT1) 转运体的 mRNA 丰度。采用 JMP Pro11 软件进行方差分析。雄性小鸡的 bo,+AT、EAAT3、ASCT1、y+LAT2 和 GLUT2 mRNA 丰度大于雌性小鸡（P < 0.05）。其他 5 种转运体和 APN 在雄性和雌性之间的表达无差异。bo,+AT、PepT1、SGLT1、ASCT1 和 y+LAT2 mRNA 存在性别 x 年龄的互作，doh 时雄性小鸡的 mRNA 丰度大于雌性小鸡，但在第 7 天和第 14 天，雄性和雌性小鸡之间无差异。GLUT2 mRNA 存在性别 x 年龄 x 组织的 3 重互作。doh 时雄性小鸡的十二指肠和回肠及第 7 天的空肠中 GLUT2 mRNA 丰度较高，但在第 14 天，雄性和雌性小鸡之间无差异。因此，在 doh 时雄性和雌性小鸡的一些营养转运体存在差异表达，但在较晚的年龄阶段没有。
