Martyniuk Viktoria, Khoma Vira, Matskiv Tetiana, Yunko Kateryna, Gnatyshyna Lesya, Stoliar Oksana, Faggio Caterina
Ternopil Volodymyr Hnatiuk National Pedagogical University, M. Kryvonosa Str. 2, 46027 Ternopil, Ukraine.
Ternopil Volodymyr Hnatiuk National Pedagogical University, M. Kryvonosa Str. 2, 46027 Ternopil, Ukraine; I. Ya. Horbachevsky Ternopil National Medical University, Maidan Voli 1, 46001 Ternopil, Ukraine.
Environ Toxicol Pharmacol. 2023 Mar;98:104068. doi: 10.1016/j.etap.2023.104068. Epub 2023 Jan 19.
Microplastic (MP) and heating (T) suspected to modulate biological effects of aquatic contaminants. Salinomycin (Sal) is veterinary antibiotic and anticancer agent. The goal of this study was to examine the multistress effect of MP, Sal and T on the bioindicator bivalve mollusc. The Unio tumidus were treated with MP (1 mg L), Sal (0.6 µg L), their combination under 18° C (Mix) and 25° C (MixT) for 14 days. The digestive glands were analyzed. MP and Sal did not cause changes of Mn- and Cu,Zn-SOD, lipid peroxidation and Cyp-450-depended EROD levels, whereas catalase, GST and protein carbonyls (Sal-group) increased compared to control. In the Mix-group, enzymes, particularly EROD and GST (by 34% and 115% respectively) were up-regulated. However, in the MixT-group, they were corresponding to control or lesser (EROD, catalase). Our findings emphasize the need to take into account multistress interactions in the MP environmental risk assessment.
微塑料（MP）和加热（T）可能会调节水生污染物的生物效应。盐霉素（Sal）是一种兽用抗生素和抗癌剂。本研究的目的是研究MP、Sal和T对生物指示双壳贝类的多重应激效应。将膨胀珠蚌分别用MP（1毫克/升）、Sal（0.6微克/升）、它们在18℃（Mix）和25℃（MixT）下的组合处理14天。对消化腺进行分析。MP和Sal不会引起锰和铜锌超氧化物歧化酶、脂质过氧化和细胞色素P450依赖的乙氧基异吩恶唑酮-O-脱乙基酶（EROD）水平的变化，而与对照组相比，过氧化氢酶、谷胱甘肽S-转移酶和蛋白质羰基（Sal组）增加。在Mix组中，酶，特别是EROD和谷胱甘肽S-转移酶（分别增加34%和115%）上调。然而，在MixT组中，它们与对照组相当或更低（EROD、过氧化氢酶）。我们的研究结果强调在MP环境风险评估中需要考虑多重应激相互作用。
