State Key Laboratory of Targeting Oncology, National Center for International Research of Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Bio-targeting Theranostics, Guangxi Medical University, Nanning, Guangxi, 530021, China; School of Pharmacy, Guangxi Medical University, Nanning, 530021, China.
State Key Laboratory of Targeting Oncology, National Center for International Research of Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Bio-targeting Theranostics, Guangxi Medical University, Nanning, Guangxi, 530021, China.
Biosens Bioelectron. 2023 Apr 1;225:115081. doi: 10.1016/j.bios.2023.115081. Epub 2023 Jan 15.
An ultrasensitive electrochemical immunosensor based on signal amplification of the deposition of the electroactive ferrocene-tyramine (Fc-Tyr) molecule, catalyzed by horseradish peroxidase (HRP), was constructed for the detection of the liver cancer marker Glypican-3 (GPC3). Functional electroactive molecule Fc-Tyr is reported to exhibit both the enzymatic cascade catalytic activity of tyramine signal amplification (TSA) and the excellent redox properties of ferrocene. In terms of design, the low matrix effects inherent in using the magnetic bead platforms, a quasi-homogeneous system, allowed capturing the target protein GPC3 without sample pretreatment, and loading HRP to trigger the TSA, which induced a large amount of Fc-Tyr deposited on the electrode surface layer by layer as a signal probe for the detection of GPC3. The concept of Fc-Tyr as an electroactive label was validated, GPC3 biosensor exhibited high selectivity and sensitivity to GPC3 in the range of 0.1 ng mL-1 μg mL. Finally, the sensor was used simultaneously with ELISA to assess GPC3 levels in the serum of clinical liver cancer patients, and the results showed consistency, with a recovery of 98.33-105.35% and a relative standard deviation (RSD) of 4.38-8.18%, providing a theoretical basis for achieving portable, rapid and point of care testing (POCT) of tumor markers.
基于辣根过氧化物酶（HRP）催化的电化学活性二茂铁-酪胺（Fc-Tyr）分子沉积的信号放大，构建了一种用于检测肝癌标志物磷脂酰聚糖-3（GPC3）的超灵敏电化学免疫传感器。报道称，功能型电化学活性分子 Fc-Tyr 同时具有酪胺信号放大（TSA）的酶级联催化活性和二茂铁的优异氧化还原性能。在设计方面，使用磁珠平台固有的低基质效应（一种准均相体系）允许在无需样品预处理的情况下捕获靶蛋白 GPC3，并加载 HRP 以触发 TSA，这导致大量 Fc-Tyr 逐层沉积在电极表面作为检测 GPC3 的信号探针。验证了 Fc-Tyr 作为电活性标记物的概念，GPC3 生物传感器在 0.1ng/mL-1μg/mL 的范围内对 GPC3 表现出高选择性和灵敏度。最后，该传感器与 ELISA 一起用于评估临床肝癌患者血清中的 GPC3 水平，结果显示具有一致性，回收率为 98.33-105.35%，相对标准偏差（RSD）为 4.38-8.18%，为实现肿瘤标志物的便携、快速和即时检测（POCT）提供了理论依据。
