Anchidin-Norocel Liliana, Savage Wesley K, Gheorghita Roxana, Amariei Sonia
Faculty of Medicine and Biological Sciences, Stefan cel Mare University of Suceava, 720229 Suceava, Romania.
Integrated Center for Research, Development and Innovation in Advanced Materials, Nanotechnologies, and Distributed Systems for Fabrication and Control, Stefan cel Mare University of Suceava, 720229 Suceava, Romania.
Micromachines (Basel). 2023 Jul 30;14(8):1529. doi: 10.3390/mi14081529.
Food is humans' main source of nickel intake, which is responsible for the prevalence of allergic contact dermatitis and other pathological afflictions. While robust, the classical methods for nickel detection-atomic absorption spectrometry and inductively coupled plasma mass spectrometry-are expensive and laborious; in contrast, modern methods that utilize sensors-of which most are electrochemical-have rapid run times, are cost-effective, and are easily assembled. Here, we describe the use of four biopolymers (alginate, agar, chitosan, and carrageenan) for receptor immobilization on biosensors to detect nickel ions and use an optimization approach with three biopolymer concentrations to assay analytical performance profiles. We measured the total performance of screen-printed carbon electrodes immobilized with the biopolymer-sensor combinations using cyclic voltammetry (CV). Voltammetric behavior favored the carrageenan biosensor, based on performance characteristics measured using CV, with sensitivities of 2.68 (for 1% biopolymer concentration) and 2.08 (for 0.5% biopolymer concentration). Our results indicated that among the four biopolymer combinations, carrageenan with urease affixed to screen-printed electrodes was effective at coupling for nickel detection.
食物是人类镍摄入的主要来源，这导致了过敏性接触性皮炎和其他病理疾病的流行。虽然经典的镍检测方法——原子吸收光谱法和电感耦合等离子体质谱法——很可靠，但它们昂贵且费力；相比之下，利用传感器的现代方法——其中大多数是电化学方法——运行时间短、成本效益高且易于组装。在这里，我们描述了使用四种生物聚合物（海藻酸盐、琼脂、壳聚糖和角叉菜胶）将受体固定在生物传感器上以检测镍离子，并使用三种生物聚合物浓度的优化方法来分析分析性能概况。我们使用循环伏安法（CV）测量了固定有生物聚合物 - 传感器组合的丝网印刷碳电极的整体性能。基于使用CV测量的性能特征，伏安行为有利于角叉菜胶生物传感器，其灵敏度分别为2.68（生物聚合物浓度为1%时）和2.08（生物聚合物浓度为0.5%时）。我们的结果表明，在四种生物聚合物组合中，固定有脲酶的角叉菜胶与丝网印刷电极耦合用于镍检测是有效的。
