College of Plant Protection, Anhui Agricultural University, Hefei 230036, Anhui, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Agric Food Chem. 2023 Feb 1;71(4):1837-1844. doi: 10.1021/acs.jafc.2c06132. Epub 2023 Jan 22.
Odorant receptors (ORs) in insects are crucial for the detection of chemical signals. However, the functions of the conserved OR genes among insect species are rarely studied. In this study, we analyzed a well-conserved OR clade in Diptera insects and cloned a gene from this clade, , in the hoverfly . Real-time quantitative PCR showed that was highly expressed in the antennae and upregulated in the mated females, and functional characterization showed that EcorOR4 was narrowly tuned to 1-octen-3-ol. Electroantennogram assays revealed that the antennal response of mated females to 1-octen-3-ol was significantly higher than that of mated males, but no significant differences were observed between male and female virgins. Finally, a Y-tube olfactometer bioassay showed that 1-octen-3-ol is an attractant for only mated female adults. These results demonstrate that EcorOR4 is involved in the detection of 1-octen-3-ol and that this compound may affect the host-finding and oviposition behavior in female .
昆虫的气味受体（ORs）对于化学信号的检测至关重要。然而，昆虫种间保守的 OR 基因的功能很少被研究。在这项研究中，我们分析了双翅目昆虫中一个高度保守的 OR 分支，并从这个分支中克隆了一个基因，在食蚜蝇 中。实时定量 PCR 显示， 在触角中高度表达，并在交配后的雌性中上调表达，功能表征表明 EcorOR4 对 1-辛烯-3-醇有较窄的调谐。触角电生理学测定显示，交配后的雌性对 1-辛烯-3-醇的触角反应明显高于交配后的雄性，但雄性和雌性处女之间没有观察到显著差异。最后，Y 型嗅觉仪生物测定表明，1-辛烯-3-醇仅对交配后的雌性成虫具有吸引力。这些结果表明，EcorOR4 参与了 1-辛烯-3-醇的检测，而这种化合物可能影响雌性的宿主寻找和产卵行为。
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