Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400716, China.
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Commun Biol. 2023 Feb 15;6(1):176. doi: 10.1038/s42003-023-04551-5.
The oriental fruit fly Bactrocera dorsalis (Hendel) is a notorious pest of fruit crops. Gravid females locate suitable oviposition sites by detecting host plant volatiles. Here, we demonstrate that 1-octen-3-ol, a volatile from mango, guides the oviposition behavior of female flies. Two odorant receptors (BdorOR7a-6 and BdorOR13a) are identified as key receptors for 1-octen-3-ol perception by qPCR analysis, heterologous expression in Xenopus laevis oocytes and HEK 293 cells followed by in vitro binding assays, as well as CRISPR/Cas9 genome editing in B. dorsalis. Molecular docking and site-directed mutagenesis are used to determine major binding sites for 1-octen-3-ol. Our results demonstrate the potential of 1-octen-3-ol to attract gravid females and molecular mechanism of its perception in B. dorsalis. BdorOR7a-6 and BdorOR13a can therefore be used as molecular targets for the development of female attractants. Furthermore, our site-directed mutagenesis data will facilitate the chemical engineering of 1-octen-3-ol to generate more efficient attractants.
东方果实蝇 Bactrocera dorsalis（Hendel）是一种臭名昭著的水果作物害虫。受孕雌蝇通过检测寄主植物挥发物来定位合适的产卵部位。在这里，我们证明了芒果中的 1-辛烯-3-醇是引导雌蝇产卵行为的挥发性物质。通过 qPCR 分析、异源表达在非洲爪蟾卵母细胞和 HEK293 细胞中的体外结合试验，以及 CRISPR/Cas9 基因组编辑，鉴定了两种气味受体（BdorOR7a-6 和 BdorOR13a）作为 1-辛烯-3-醇感知的关键受体。分子对接和定点突变用于确定 1-辛烯-3-醇的主要结合位点。我们的结果证明了 1-辛烯-3-醇吸引受孕雌蝇的潜力及其在 B. dorsalis 中的感知分子机制。BdorOR7a-6 和 BdorOR13a 因此可以用作开发雌性引诱剂的分子靶标。此外，我们的定点突变数据将有助于 1-辛烯-3-醇的化学工程改造，以产生更有效的引诱剂。
