Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
Department of Toxicology, School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Medical College of Soochow University, Suzhou 215123, Jiangsu, China.
Ecotoxicol Environ Saf. 2023 Feb;251:114565. doi: 10.1016/j.ecoenv.2023.114565. Epub 2023 Jan 20.
Microplastics (MPs) and bisphenol AF (BPAF) are two environmental pollutants that usually coexist in the natural environment. Studies of MPs or BPAF have gradually increased in recent years, but few studies have focused on the combination toxic effects. In this study, the subchronic model of adult zebrafish was exposed to 1 mg/L nanolevel microplastics and 200 μg/L BPAF for 45 days; the parental zebrafish were spawning every 3 days during exposure, and the effects of continuous poisoning were examined on the offspring after 1-9 spawns. The results showed that single BPAF exposure or BPAF and nanoplastic coexposure can both decrease the number of eggs laid and the locomotor behavior of parental zebrafish and impact the hatching rate, mortality, body length and locomotor behavior of offspring zebrafish, especially in 7-9 spawn. BPAF were accumulated in parental zebrafish intestinal in 334.62 ng/g in BPAF group and 594.52 ng/g in nm+BPAF group, and accumulated in whole offspring zebrafish for 281.6 ng/g in BPAF group and 321.46 ng/g in nm+BPAF group. Neurodevelopmental, inflammation, apoptosis and oxidative stress-related genes were also significantly increased after 7-9 spawn. In addition, the exacerbated accumulation in the BPAF+nm group in parental and offspring zebrafish may be the reason for the accelerated toxic effect in the present research. In this study, we investigated the combined effects of nanoplastics and BPAF on parental and offspring zebrafish in the aquatic environment to identify the accumulative toxic effects and provide new experimental support for assessing the effects of coexposure on aquatic organisms.
微塑料 (MPs) 和双酚 AF (BPAF) 是两种通常共存于自然环境中的环境污染物。近年来，对 MPs 或 BPAF 的研究逐渐增加，但很少有研究关注它们的联合毒性作用。在这项研究中，成年斑马鱼的亚慢性模型暴露于 1mg/L 的纳米级微塑料和 200μg/L 的 BPAF 中 45 天；在暴露期间，亲代斑马鱼每 3 天产卵一次，并在 1-9 次产卵后检查连续中毒对后代的影响。结果表明，单独暴露于 BPAF 或 BPAF 和纳米塑料共同暴露均可降低亲代斑马鱼产卵量和运动行为，并影响子代斑马鱼的孵化率、死亡率、体长和运动行为，尤其是在第 7-9 次产卵。BPAF 在 BPAF 组中亲代斑马鱼肠道内积累了 334.62ng/g，在 nm+BPAF 组中积累了 594.52ng/g，在 BPAF 组中在整个子代斑马鱼内积累了 281.6ng/g，在 nm+BPAF 组中积累了 321.46ng/g。神经发育、炎症、凋亡和氧化应激相关基因在第 7-9 次产卵后也显著增加。此外，亲代和子代斑马鱼中 BPAF+nm 组的积累加剧可能是本研究中加速毒性作用的原因。在这项研究中，我们研究了纳米塑料和 BPAF 对水生环境中亲代和子代斑马鱼的联合作用，以确定累积毒性作用，并为评估共暴露对水生生物的影响提供新的实验支持。
