College of Advanced Agricultural Sciences, Zhejiang A&F University, Lin'an, Hangzhou 311300, PR China.
Yunnan Tobacco Quality Inspection & Supervision Station, Kunming 650106, PR China.
Int J Food Microbiol. 2023 Mar 2;388:110089. doi: 10.1016/j.ijfoodmicro.2023.110089. Epub 2023 Jan 13.
Botrytis cinerea is a phytopathogenic fungus that causes gray mold, a major postharvest disease of fruits and vegetables. Chemical fungicides remain the main solution to control Botrytis disease, but concerns have raised about their safety to environment and human health, and there is an increasing need for development of more effective and less toxic treatments. In this study the divalent cation chelating agent ethylenediaminetetraacetic acid (EDTA) exhibited marked antifungal activity against B. cinerea, including inhibition of spore germination, mycelial growth, infection cushion formation, stimulation of cell death, and impairment of fungal virulence. These adverse effects of EDTA could be reversed by the addition of calcium ion, implying that metal ion chelation is involved in the fungicidal mechanism. Bean leaf and tomato fruit protection assay indicated that EDTA treatment led to a significant reduction of infection by B. cinerea. Furthermore, the antifungal activity of EDTA was significantly enhanced when used in combination with fenhexamid. These findings suggest that EDTA could be a promising tool to control B. cinerea, and application of EDTA may reduce the use of conventional chemical fungicides.
葡萄孢菌是一种植物病原真菌，可引起灰霉病，这是水果和蔬菜的一种主要采后病害。化学杀菌剂仍然是控制灰霉病的主要方法，但人们对其对环境和人类健康的安全性表示担忧，因此越来越需要开发更有效和毒性更低的处理方法。在这项研究中，二价阳离子螯合剂乙二胺四乙酸（EDTA）对葡萄孢菌表现出明显的抗真菌活性，包括抑制孢子萌发、菌丝生长、感染垫形成、刺激细胞死亡和损害真菌毒力。EDTA 的这些不利影响可以通过添加钙离子来逆转，这表明金属离子螯合参与了杀菌机制。豆叶和番茄果实保护试验表明，EDTA 处理可显著减少葡萄孢菌的感染。此外，当 EDTA 与苯并烯菌胺联合使用时，其抗真菌活性显著增强。这些发现表明，EDTA 可能是控制葡萄孢菌的一种有前途的工具，并且 EDTA 的应用可能会减少传统化学杀菌剂的使用。
