Luck M R, Jungclas B
Institute for Hormone and Fertility Research, Hamburg, Federal Republic of Germany.
J Endocrinol. 1987 Sep;114(3):423-30. doi: 10.1677/joe.0.1140423.
The effects of catecholamines and ascorbic acid on cultured bovine granulosa cells have been examined to assess their possible role in the initiation and maintenance of luteal oxytocin secretion. The actions of these agents have also been compared with the previously reported ability of follicular theca tissue to enhance oxytocin secretion. Using granulosa cells cultured in serum-supplemented medium, we observed a highly significant enhancement of oxytocin secretion in the presence of adrenaline and noradrenaline, particularly over the concentration range 1-10 mumol/l. This effect was accompanied by smaller and less consistent changes in progesterone secretion and did not involve any change in the time-course of oxytocin secretion. Acetylcholine was without effect. Ascorbic acid stimulated oxytocin secretion when used alone over a range of concentrations, but was also able to synergize with adrenaline. Lactic acid was ineffective. The stimulation of oxytocin secretion by adrenaline could be blocked by equimolar propranolol, but the stimulation of progesterone was not blocked. Propranolol had a variable effect on the ability of theca tissue to stimulate oxytocin secretion by granulosa cells but the results also suggested the presence of some beta-agonistic activity in the culture medium. We conclude, first, that catecholamines may be involved in the regulation of ovarian oxytocin secretion, secondly, that ascorbate may regulate oxytocin secretion through its involvement in the biosynthesis of oxytocin but also through interaction with catecholamines and, thirdly, that the stimulatory action of theca tissue probably does not involve the action of beta-agonists.
已研究了儿茶酚胺和抗坏血酸对培养的牛颗粒细胞的作用，以评估它们在黄体催产素分泌的启动和维持中可能发挥的作用。还将这些物质的作用与先前报道的卵泡膜组织增强催产素分泌的能力进行了比较。使用在补充血清的培养基中培养的颗粒细胞，我们观察到在肾上腺素和去甲肾上腺素存在下，催产素分泌有高度显著的增强，特别是在1 - 10 μmol/l的浓度范围内。这种作用伴随着孕酮分泌较小且不太一致的变化，并且不涉及催产素分泌时间进程的任何改变。乙酰胆碱无作用。抗坏血酸单独使用时在一定浓度范围内刺激催产素分泌，但也能够与肾上腺素协同作用。乳酸无效。肾上腺素对催产素分泌的刺激可被等摩尔的普萘洛尔阻断，但对孕酮的刺激未被阻断。普萘洛尔对卵泡膜组织刺激颗粒细胞分泌催产素的能力有可变作用，但结果也表明培养基中存在一些β - 激动活性。我们得出结论，首先，儿茶酚胺可能参与卵巢催产素分泌的调节；其次，抗坏血酸盐可能通过参与催产素的生物合成以及与儿茶酚胺的相互作用来调节催产素分泌；第三，卵泡膜组织的刺激作用可能不涉及β - 激动剂的作用。
