Webley G E, Luck M R, Hearn J P
MRC/AFRC Comparative Physiology Research Group, Institute of Zoology, London, U.K.
J Reprod Fertil. 1988 Nov;84(2):669-77. doi: 10.1530/jrf.0.0840669.
Granulosa cells, aspirated from the follicles of patients undergoing treatment for in-vitro fertilization, were cultured in serum-supplemented medium. Adrenaline and noradrenaline stimulated a dose-related increase in progesterone secretion with a maximum stimulation at 10(-5) M, a response that was prevented by the beta-antagonist, propranolol. Adrenaline and hCG showed similar characteristics in their stimulation of progesterone secretion but there was no further increase in progesterone when the 2 compounds were added together. Melatonin stimulated progesterone secretion and, like adrenaline, this stimulation was prevented by propranolol. The ability of both adrenaline and melatonin to increase progesterone secretion was dependent on the degree of follicular development, as determined by peripheral oestradiol concentrations, on the day of laparoscopy. These results suggest that adrenaline and melatonin may have a physiological role in modulating luteal function and that melatonin may act by a beta-adrenergic-related mechanism.
从接受体外受精治疗的患者卵泡中吸出的颗粒细胞，在添加血清的培养基中培养。肾上腺素和去甲肾上腺素刺激孕酮分泌呈剂量相关增加，在10(-5)M时刺激最大，β受体拮抗剂普萘洛尔可阻止这种反应。肾上腺素和人绒毛膜促性腺激素在刺激孕酮分泌方面表现出相似的特征，但当两种化合物一起添加时，孕酮没有进一步增加。褪黑素刺激孕酮分泌，与肾上腺素一样，这种刺激被普萘洛尔阻止。肾上腺素和褪黑素增加孕酮分泌的能力取决于腹腔镜检查当天外周雌二醇浓度所确定的卵泡发育程度。这些结果表明，肾上腺素和褪黑素可能在调节黄体功能方面具有生理作用，并且褪黑素可能通过与β-肾上腺素能相关的机制起作用。
